NMDAR2A Antibody

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Western Blot: NMDAR2A Antibody [NB300-105]
Immunohistochemistry: NMDAR2A Antibody [NB300-105] - Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

NMDAR2A Antibody Summary

Immunogen
Fusion protein from the C-terminal region of the NR2A subunit
Localization
NB 300-105 can be used to demonstrate that both the NR2A is highly enriched in hippocampus compared to either cortex or cerebellum.
Specificity
Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin 1:1000- 1:2000
  • Immunoprecipitation 3 ul per 200 ug lysate
Application Notes
NB 300-105 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2A.
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-105 in the following applications:

  • IHC
    1 publication
  • IP
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)

Alternate Names for NMDAR2A Antibody

  • glutamate receptor, ionotropic, N-methyl D-aspartate 2A
  • hNR2A
  • NMDA receptor subtype 2A
  • N-methyl D-aspartate receptor subtype 2A
  • N-methyl-D-aspartate receptor subunit 2A
  • subunit epsilon-1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMDAR2A Antibody (NB300-105)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IP, WB.


Filter By Application
IHC
(1)
IP
(1)
WB
(5)
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Human
(1)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using NB300-105 Applications Species
Wang X, Zhao L, Zhang J et al. Requirement for Microglia for the Maintenance of Synaptic Function and Integrity in the Mature Retina. J Neurosci 2016 Mar 2 [PMID: 26937019] (IHC) IHC
Lo SC, Wang Y, Weber M et al. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control J. Neurosci. 2015 Feb 04 [PMID: 25653368] (WB, Mouse)

Details:
NMDAR2A (GluN2A/NR2A) antibody used for WB analysis of the lysates from hippocampus tissues which were collected from experienced mice (11 months of age; tissues collected 1 month after the last behavior testing ) from WT and Casp3?/? mice genotypes previously subjected to repeated challenges with new behavioral testing (Figure 7B).
WB Mouse
Hanson JE, Pare J, Deng L et al. Altered GluN2B NMDA receptor function and synaptic plasticity during early pathology in the PS2APP mouse model of Alzheimer's disease. Neurobiol. Dis. 2014 Dec 04 [PMID: 25484285] (WB, Mouse)

Details:
NMDAR2A antibody used for WB at 1:1000 dilution on brain lysates from male mice homozygous for the PS2APP transgenes (mouse model of Alzheimer's disease) and their wt littermate controls.
WB Mouse
Das SR, Magnusson KR. Changes in expression of splice cassettes of NMDA receptor GluN1 subunits within the frontal lobe and memory in mice during aging. Behav Brain Res;222(1):122-133. 2011 Sep 12. [PMID: 21443909] (WB, Mouse) WB Mouse
Karolewicz B, Szebeni K, Gilmore T et al. Elevated levels of NR2A and PSD-95 in the lateral amygdala in depression. Int J Neuropsychopharmacol 2009 Mar [PMID: 18570704] (WB, Human) WB Human
Ning, K et al. Dual Neuroprotective Signaling Mediated by Downregulating Two Distinct Phosphatase Activities of PTEN. The Journal of Neuroscience 24: 4052-4060. 2004 [PMID: 15102920] (WB, IP, Rat) WB, IP Rat

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Bioinformatics

Gene Symbol GRIN2A
Entrez
Uniprot