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Glutamate Receptor 4 Antibody

Images

 
Western Blot: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Glutamate Receptor 4 Antibody Summary

Immunogen
Peptide with sequence C-KKLDQREYPGSETP corresponding to internal region according to NP_001070711.1, NP_001070712.1.
Epitope
internal region
Specificity
This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRIA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 2 ug/mL
Application Notes
WB: Approx. 90 kDa band observed in human brain (cerebellum) lysates (calculated MW of 99.2 kDa band according to NP_001070711.1). IHC-P: Human cortex shows cytoplasm staining of neuronal cells.
Publications
Read Publication using NBP1-02312.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glutamate Receptor 4 Antibody

  • AMPA-selective glutamate receptor 4
  • GluA4
  • GluR4
  • gluR-4
  • GLUR4C
  • GLUR4gluR-D
  • GLURD
  • GluR-D
  • glutamate receptor 4
  • Glutamate receptor ionotropic, AMPA 4
  • glutamate receptor, ionotrophic, AMPA 4

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutamate Receptor 4 Antibody (NBP1-02312)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GRIA4
Entrez
Uniprot