Glutamate Receptor 4 Antibody

Western Blot: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glutamate Receptor 4 Antibody Summary

• Immunogen: Peptide with sequence C-KKLDQREYPGSETP corresponding to internal region according to NP_001070711.1, NP_001070712.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 2 ug/mL
• Application Notes: WB: Approx. 90 kDa band observed in human brain (cerebellum) lysates (calculated MW of 99.2 kDa band according to NP_001070711.1). IHC-P: Human cortex shows cytoplasm staining of neuronal cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutamate Receptor 4 Antibody

• AMPA-selective glutamate receptor 4
• GluA4
• GluR4
• gluR-4
• GLUR4C
• GLUR4gluR-D
• GLURD
• GluR-D
• glutamate receptor 4
• Glutamate receptor ionotropic, AMPA 4
• glutamate receptor, ionotrophic, AMPA 4

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutamate Receptor 4 Antibody (NBP1-02312)(1)

Publication using NBP1-02312

• Earnshaw BA, Bressloff PC. Biophysical model of AMPA receptor trafficking and its regulation during long-term potentiation/long-term depression. J Neurosci 2006-11-22 [PMID: 17122061]

Bioinformatics

• Gene Symbol: GRIA4
• Entrez:
  • 2893(Human)
  • 14802(Mouse)
  • 29629(Rat)
• Uniprot:
  • NP_000820.3(Human)