JMY Antibody

Western Blot: JMY Antibody [NBP3-23402] - Western blot showing JMY expression in rat PC12 cells (lane 1), human Jurkat cells (lane 2), and adult mouse heart (lane 3). The blots were probed with anti-JMY (C-terminal region) rabbit polyclonal antibody at 1:500.

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

JMY Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: JMY synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse JMY. This sequence is highly conserved in human, rat, frog, and zebrafish JMY proteins, and has low homology to other WASP family members. [UniProt# Q9QXM1]
• Specificity: The antibody detects a 130 kDa protein corresponding to the molecular mass of JMY on SDS-PAGE immunoblots of human HeLa, Jurkat cells, rat PC12 cells, and mouse brain and heart.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JMY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for JMY Antibody

• FLJ37870
• junction mediating and regulatory protein, p53 cofactor
• junction-mediating and regulatory protein
• junction-mediating and -regulatory protein
• MGC163496
• WAS protein homology region 2 domain containing 1-like 3
• WHDC1L3

Background

Cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependenttranscription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JMY