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SRFBP1 Antibody

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Western Blot: SRFBP1 Antibody [NBP1-88518] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Western Blot: SRFBP1 Antibody [NBP1-88518] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: SRFBP1 Antibody [NBP1-88518] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
Immunohistochemistry-Paraffin: SRFBP1 Antibody [NBP1-88518] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SRFBP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKAAVQAFKEARQNVAEVESSKNASEDNHSENTLYSNDNGSNLQREATVISEQKVKETKILAKKPIHNSKEKIAKMEHGPKAVTIANSPSKP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRFBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SRFBP1 Protein (NBP1-88518PEP)
Publications
Read Publication using
NBP1-88518 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SRFBP1 Antibody

  • BUD22 homolog
  • BUD22
  • FLJ25286
  • P49
  • p49/STRAP
  • Rlb1
  • serum response factor binding protein 1
  • serum response factor-binding protein 1
  • SRF-dependent transcription regulation associated protein
  • SRF-dependent transcription regulation-associated protein
  • STRAP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
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Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a dilution of 1:1,000 (1ug/ml) (right) . Detection: DAB
Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.
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Western Blot: ARHGEF7 Antibody [NBP1-88650] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: ARHGEF7 Antibody [NBP1-88650] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ARHGEF7 Antibody [NBP1-88650] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
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Flow Cytometry: ASCC1 Antibody (OTI6G5) [NBP2-01345] - ASCC1 Antibody (6G5) [NBP2-01345] - Analysis of Jurkat cells, using anti-ASCC1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ASCC1 Antibody (OTI6G5) [NBP2-01345] - ASCC1 Antibody (6G5) [NBP2-01345] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASCC1 antibody, and then analyzed by Flow cytometry.
Western Blot: ASCC1 Antibody (OTI6G5) [NBP2-01345] - ASCC1 Antibody (6G5) [NBP2-01345] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC1.
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ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Immunofluorescence indicates NF-kB p65 subunit was expressed exclusively in AA-G groups and AST-G cytoplasm. High glucose stimulation impact on TSMCs in NF-kB pathway. (A) NF-kB p65 protein subunit expression in TSMCs in each group. (B) NF-kB p65 subunit immunofluorescence. Each number denotes different subunits. Alexa 488 green served as secondary antibodies.Citation: Hara K, Hamada C, Wakabayashi K, Kanda R, Kaneko K, Horikoshi S, et al. (2017) Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells. PLoS ONE 12(9): e0184332. https://doi.org/10.1371/journal.pone.0184332
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Standard curve using the internal RelA standard.
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Nuclear translocation of NF-kB p65 with LPS in Jurkat cells. Jurkat cells, grown to a density of 2X10(6) cells/ml, were treated with LPS (15 ug/ml). The cells were harvested at various indicated time points, and cell lysates were tested in Western analysis using NB100-56712 and also in this ELISA Kit.
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Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Simple Western lane view shows lysates of CH‑1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse c‑Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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AF2428
Ficolin-2 Antibody
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Western Blot: CDCA5 Antibody [NBP1-89530] - Analysis in human cell line SCLC-21H.
Immunohistochemistry-Paraffin: CDCA5 Antibody [NBP1-89530] - Staining of human bone marrow shows immune cell positivity in hematopoietic cells.
Immunocytochemistry/Immunofluorescence: CDCA5 Antibody [NBP1-89530] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
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Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.
Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.
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Immunohistochemistry-Paraffin: Uroplakin IIIB Antibody [NBP1-92564] - Staining of human urninary bladder shws strong postivity in apical membrane in urothelial cells.
Immunohistochemistry-Paraffin: Uroplakin IIIB Antibody [NBP1-92564] - Staining of human lung shows moderate membranous and cytoplasmic psotivity in macrophages.
Immunohistochemistry-Paraffin: Uroplakin IIIB Antibody [NBP1-92564] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
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GCHFR Antibody
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Western Blot: GCHFR Antibody [NBP1-91931] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Western Blot: GCHFR Antibody [NBP1-91931] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: GCHFR Antibody [NBP1-91931] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
IL-12
[Unconjugated]
419-ML/CF
1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 25 kDa and 43 kDa (R) and 68 kDa (NR) .
Recombinant Mouse IL-12 (Catalog # 419‑ML/CF) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01-0.1 ng/mL.
(229 Publications)
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p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(15 Publications)
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
PMEL17/SILV
Antibody (OTI10E5)
NBP1-47974
Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded breast tissue using anti-PMEL17/SILV mouse monoclonal antibody.
(1 Review)
Species: Ca, Hu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for SRFBP1 Antibody (NBP1-88518)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol SRFBP1