SRFBP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SRFBP1 Antibody [NBP1-88518] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.

Immunohistochemistry-Paraffin: SRFBP1 Antibody [NBP1-88518] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRFBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SRFBP1 Antibody - BSA Free (NBP1-88518) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SRFBP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LKAAVQAFKEARQNVAEVESSKNASEDNHSENTLYSNDNGSNLQREATVISEQKVKETKILAKKPIHNSKEKIAKMEHGPKAVTIANSPSKP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRFBP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SRFBP1 Protein (NBP1-88518PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SRFBP1 Antibody - BSA Free

• BUD22 homolog
• BUD22
• FLJ25286
• P49
• p49/STRAP
• Rlb1
• serum response factor binding protein 1
• serum response factor-binding protein 1
• SRF-dependent transcription regulation associated protein
• SRF-dependent transcription regulation-associated protein
• STRAP

Background

SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. May playa role in biosynthesis and/or processing of SLC2A4 in adiposecells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRFBP1 Antibody (NBP1-88518)(1)

Publication using NBP1-88518

• Andonegui-Elguera M A, Caceres-Gutierrez R E et al. BUB1 and SURVIVIN proteins are not degraded after a prolonged mitosis and accumulate in the nuclei of HCT116 cells. Cell Death Discov 2016-08-11 [PMID: 27818790] (WB, Human)

Bioinformatics

• Gene Symbol: SRFBP1