FMRP Antibody (OTI1C6)

Images

 
Western Blot: FMRP Antibody (1C6) [NBP2-01770] Analysis of extracts (35ug) from 9 different cell lines by using anti-FMRP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: FMRP Antibody (1C6) [NBP2-01770] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FMRP.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Human pancreas tissue using anti-FMRP mouse monoclonal antibody.
Flow Cytometry: FMRP Antibody (1C6) [NBP2-01770] - Analysis of Jurkat cells, using anti-FMRP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FMRP Antibody (1C6) [NBP2-01770] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMRP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FMRP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FMRP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FMRP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Human colon tissue using anti-FMRP mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FMRP Antibody (OTI1C6) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 36-279 of human FMR1(NP_002015) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FMR1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FMRP Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-01770 in the following applications:

Publications
Read Publication using
NBP2-01770 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for FMRP Antibody (OTI1C6)

  • FMRPFRAXAPOF
  • fragile X mental retardation 1 protein
  • fragile X mental retardation 1
  • MGC87458
  • POF1
  • premature ovarian failure 1
  • Protein FMR-1

Background

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-79842
Species: Hu, Mu
Applications: WB, IP
NBP2-84403
Species: Hu
Applications: WB
NBP1-89546
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB300-259
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-126
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-42827
Species: Hu, Mu, Rt, Ch, Fi, Re, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
NB100-56603
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
NB100-1277
Species: Hu, Mu, Ft
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
NB600-1101
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-04611
Species: Hu
NB110-39033
Species: Hu, Mu, Rt, Ch
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB400-153
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-19586
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-88191
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-13195
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for FMRP Antibody (NBP2-01770)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for FMRP Antibody (NBP2-01770) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-01770:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FMRP NBP2-01770
Enlarge
reviewed by:
Anonymous
WB Mouse 03/21/2018
View

Summary

ApplicationWestern Blot
Sample Testedcerebellum lysate
SpeciesMouse
LotA001

Comments

Commentsloaded 50ug total protein each lane. Anti-fmrp antibody used 1:1000. Washed 3 times in 0.1% PBST, 10 min each time.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FMRP Antibody (NBP2-01770) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70756AF350
Alexa Fluor 405 NBP2-70756AF405
Alexa Fluor 488 NBP2-70756AF488
Alexa Fluor 532 NBP2-70756AF532
Alexa Fluor 594 NBP2-70756AF594
Alexa Fluor 647 NBP2-70756AF647
Alexa Fluor 700 NBP2-70756AF700
Alexa Fluor 750 NBP2-70756AF750
Allophycocyanin NBP2-70756APC
Allophycocyanin/Cy7 NBP2-70756APCCY7
Biotin NBP2-70756B
DyLight 350 NBP2-70756UV
DyLight 405 NBP2-70756V
DyLight 488 NBP2-70756G
DyLight 550 NBP2-70756R
DyLight 594 NBP2-70756DL594
DyLight 650 NBP2-70756C
DyLight 680 NBP2-70756FR
DyLight 755 NBP2-70756IR
FITC NBP2-70756F
HRP NBP2-70756H
Janelia Fluor 549 NBP2-70756JF549
Janelia Fluor 646 NBP2-70756JF646
PE NBP2-70756PE
PE/Atto594 NBP2-70756PEATT594
PE/Cy5.5 NBP2-70756PECY55
PE/Cy7 NBP2-70756PECY7
PerCP NBP2-70756PCP

Additional FMRP Products

Bioinformatics Tool for FMRP Antibody (NBP2-01770)

Discover related pathways, diseases and genes to FMRP Antibody (NBP2-01770). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FMRP Antibody (NBP2-01770)

Discover more about diseases related to FMRP Antibody (NBP2-01770).
 

Pathways for FMRP Antibody (NBP2-01770)

View related products by pathway.

PTMs for FMRP Antibody (NBP2-01770)

Learn more about PTMs related to FMRP Antibody (NBP2-01770).
 

Research Areas for FMRP Antibody (NBP2-01770)

Find related products by research area.

Blogs on FMRP

There are no specific blogs for FMRP, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
03/21/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FMR1
Entrez
COVID-19 update