Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Images

 
Genetic Strategies: Knockout Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IDH1 knockout ...read more
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenasemonoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by ...read more
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Endometrium tissue.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Isocitrate dehydrogenase(Right lane) cDNA for 48 hrs and ...read more
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded breast tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded endometrium tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clone
OTI2H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human IDH1 expression vector
Specificity
This antibody is specific for Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDH1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Knockout Validated
Theoretical MW
46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Isocitrate Dehydrogenase 1/IDH1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47804 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

  • Cytosolic NADP-isocitrate dehydrogenase
  • EC 1.1.1.42
  • IDCD
  • IDH
  • IDH1
  • IDP
  • IDPC
  • isocitrate dehydrogenase [NADP] cytoplasmic
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • Isocitrate Dehydrogenase 1
  • NADP(+)-specific ICDH
  • NADP-dependent isocitrate dehydrogenase, cytosolic
  • NADP-dependent isocitrate dehydrogenase, peroxisomal
  • Oxalosuccinate decarboxylase
  • PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22166
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB110-61646
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-42210
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-59451
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP2-32104
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
H00171023-M05
Species: Hu
Applications: WB, ELISA, IP, S-ELISA
NBP1-32259
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-89559
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85871
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87069
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
NB120-13888
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-WhMt
NBP1-26612
Species: Hu
Applications: IP (-), WB
NBP2-74045
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-37497
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-47804
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KO

Publications for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804) (0)

There are no publications for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47804:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Isocitrate Dehydrogenase 1/IDH1 NBP1-47804
Enlarge
4
reviewed by:
Hongxia Hu
WB Human 09/12/2012
View

Summary

ApplicationWestern Blot
Sample TestedHep3B WCL
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70988AF350
Alexa Fluor 405 NBP2-70988AF405
Alexa Fluor 488 NBP2-70988AF488
Alexa Fluor 532 NBP2-70988AF532
Alexa Fluor 594 NBP2-70988AF594
Alexa Fluor 647 NBP2-70988AF647
Alexa Fluor 700 NBP2-70988AF700
Alexa Fluor 750 NBP2-70988AF750
Biotin NBP2-70988B
DyLight 350 NBP2-70988UV
DyLight 405 NBP2-70988V
DyLight 488 NBP2-70988G
DyLight 550 NBP2-70988R
DyLight 594 NBP2-70988DL594
DyLight 650 NBP2-70988C
DyLight 680 NBP2-70988FR
DyLight 755 NBP2-70988IR
FITC NBP2-70988F
HRP NBP2-70988H
Janelia Fluor 549 NBP2-70988JF549
Janelia Fluor 646 NBP2-70988JF646

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Bioinformatics Tool for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804)

Discover related pathways, diseases and genes to Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804)

Discover more about diseases related to Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804).
 

Pathways for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804)

View related products by pathway.

PTMs for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804)

Learn more about PTMs related to Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804).
 

Research Areas for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804)

Find related products by research area.

Blogs on Isocitrate Dehydrogenase 1/IDH1

There are no specific blogs for Isocitrate Dehydrogenase 1/IDH1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol IDH1
Entrez
Uniprot