Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

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Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have ...read more
Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.
ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
7G8A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDH1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Western Blot 1:500-1:2000
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) - BSA Free

  • Cytosolic NADP-isocitrate dehydrogenase
  • EC 1.1.1.42
  • IDCD
  • IDH
  • IDH1
  • IDP
  • IDPC
  • isocitrate dehydrogenase [NADP] cytoplasmic
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • Isocitrate Dehydrogenase 1
  • NADP(+)-specific ICDH
  • NADP-dependent isocitrate dehydrogenase, cytosolic
  • NADP-dependent isocitrate dehydrogenase, peroxisomal
  • Oxalosuccinate decarboxylase
  • PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IDH1