Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed ...read more
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-59680] - IHC analysis of a formalin fixed and paraffin embedded human kidney tissue section (NBP1-78269) using 1:200 dilution of Irisin/FNDC5 antibody. The ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Irisin/FNDC5 Antibody
fibronectin type III domain containing 5
fibronectin type III domain-containing protein 5
FRCP2Fibronectin type III repeat-containing protein 2
Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise. Irisin induces expression of peroxisome proliferator-activated receptor gamma, co-activator PGC1 alpha and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes. A similar function in humans is suggested. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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