Irisin/FNDC5 Antibody

Images

 
Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed ...read more
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-59680] - IHC analysis of a formalin fixed and paraffin embedded human kidney tissue section (NBP1-78269) using 1:200 dilution of Irisin/FNDC5 antibody. The ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Irisin/FNDC5 Antibody Summary

Immunogen
Partial recombinant protein made to an internal region of the human Irisin/FNDC5 protein (amino acids 32-143) [UniProt Q8NAU1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FNDC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This antibody was tested in WB on recombinant Irisin protein.
Publications
Read Publications using
NBP2-59680 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Irisin/FNDC5 Antibody

  • fibronectin type III domain containing 5
  • fibronectin type III domain-containing protein 5
  • FNDC5
  • FRCP2
  • FRCP2Fibronectin type III repeat-containing protein 2
  • Irisin

Background

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise. Irisin induces expression of peroxisome proliferator-activated receptor gamma, co-activator PGC1 alpha and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes. A similar function in humans is suggested. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Irisin/FNDC5 Antibody (NBP2-59680)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-59680 Applications Species
Bi J, Zhang J, Ren Y et al. Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin alpha V beta 5 receptor J. Cell. Mol. Med. Nov 7 2019 [PMID: 31701659] (IHC, Human) IHC Human
Kirbas A, Karakus E, Ozkanlar S et al. FNDC5 Gene Expression in the Smooth Muscles of Dromedary Camels (Camelusdromedarius) Int J Vet Sci Jan 1 2020

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Product General Protocols

View specific protocols for Irisin/FNDC5 Antibody (NBP2-59680): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Irisin/FNDC5 Antibody (NBP2-59680) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59680AF350
Alexa Fluor 405 NBP2-59680AF405
Alexa Fluor 488 NBP2-59680AF488
Alexa Fluor 532 NBP2-59680AF532
Alexa Fluor 594 NBP2-59680AF594
Alexa Fluor 647 NBP2-59680AF647
Alexa Fluor 700 NBP2-59680AF700
Alexa Fluor 750 NBP2-59680AF750
Biotin NBP2-59680B
DyLight 350 NBP2-59680UV
DyLight 405 NBP2-59680V
DyLight 488 NBP2-59680G
DyLight 550 NBP2-59680R
DyLight 594 NBP2-59680DL594
DyLight 650 NBP2-59680C
DyLight 680 NBP2-59680FR
DyLight 755 NBP2-59680IR
FITC NBP2-59680F
HRP NBP2-59680H
Janelia Fluor 549 NBP2-59680JF549
Janelia Fluor 646 NBP2-59680JF646

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Bioinformatics Tool for Irisin/FNDC5 Antibody (NBP2-59680)

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Diseases for Irisin/FNDC5 Antibody (NBP2-59680)

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Research Areas for Irisin/FNDC5 Antibody (NBP2-59680)

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Bioinformatics

Gene Symbol FNDC5