Irisin/FNDC5 Antibodies

MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." alt="HepG2 human hepatocarcinoma cell line was stained with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm of muscle cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm of muscle cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Irisin/FNDC5 was detected in immersion fixed paraffin-embedded sections of Human Heart using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94202) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Irisin/FNDC5 was detected in immersion fixed CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94201) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />