IRF3 Antibody - BSA Free

Western Blot: IRF3 Antibody [NBP1-76597] - Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody - BSA Free [NBP1-76597] - Immunofluorescence of IRF3 in Mouse Kidney cells with IRF3 antibody at 20 ug/mL.

Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-76597] - Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRF3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IRF3 Antibody - BSA Free (NBP1-76597) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3. The immunogen is located within amino acids 330 - 380 of IRF3. Amino Acid Squence: GESWPQDQPWTKRL
• Specificity: At least three isoforms of IRF3 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRF3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRF3 Peptide (NBP1-76597PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRF3 Antibody - BSA Free

• interferon regulatory factor 3
• IRF3
• IRF-3

Background

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF3
• Uniprot:
  • NP_001562(Human)