MuRF1/TRIM63 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) ...read more
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MuRF1/TRIM63 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MuRF1/TRIM63
Met1-Gly325
Accession # Q969Q1
Specificity
Detects human, mouse, and rat MuRF1/TRIM63 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM63
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5366 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MuRF1/TRIM63 Antibody [Unconjugated]

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

TRIM63 (Tripartite motif-containing protein 63; also MURF-1, SMRZ and RING finger protein 28) is a 41 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is a striated muscle protein that is found in both cytoplasm and nucleus. TRIM63 has multiple finctions, among which are the inhibition of PKC epsilon -mediated cardiomyocyte hypertrophy and the maintenance of skeletal muscle M-line integrity. Human TRIM63 is 353 amino acids (aa) in length. It contains one RING finger domain (aa 23‑82), a B-Box type zinc-finger region (aa 117‑159), a coiled-coil region (aa 207‑269) and a C-terminal COS domain. Isoforms of TRIM63 show one potential alternate start site at Met14, a deletion of aa 105‑132 and a 21 aa substitution for aa 326‑353. Over aa 1‑325, human TRIM63 exhibits 93% aa identity with mouse TRIM63.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MuRF1/TRIM63 Antibody (AF5366)(11)

We have publications tested in 4 confirmed species: Mouse, Rat, Porcine, Zebrafish.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(11)
All Applications
Filter By Species
Mouse
(6)
Rat
(3)
Porcine
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF5366 Applications Species
DG Campelj, CA Timpani, AC Petersen, A Hayes, CA Goodman, E Rybalka The Paradoxical Effect of PARP Inhibitor BGP-15 on Irinotecan-Induced Cachexia and Skeletal Muscle Dysfunction Cancers, 2020;12(12):. 2020 [PMID: 33348673] (Western Blot, Mouse) Western Blot Mouse
M Noguchi, T Kitakaze, Y Kobayashi, K Mukai, N Harada, R Yamaji &beta-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice Nutrients, 2020;12(8):. 2020 [PMID: 32708051] (Western Blot, Mouse) Western Blot Mouse
P Xia, J Chen, Y Liu, M Fletcher, BC Jensen, Z Cheng Doxorubicin induces cardiomyocyte apoptosis and atrophy through cyclin-dependent kinase 2-mediated activation of forkhead box O1 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32075913] (Western Blot, Mouse) Western Blot Mouse
L Zhou, Y Huang, H Xie, X Mei Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 2017;0(0):. 2017 [PMID: 29257347] (Western Blot, Rat) Western Blot Rat
H Shimizu, AD Langenbach, J Huang, K Wang, G Otto, R Geisler, Y Wang, JN Chen The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes Elife, 2017;6(0):. 2017 [PMID: 28826496] (Western Blot, Zebrafish) Western Blot Zebrafish
Apichart Suksamrarn Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy In Vivo, 2016;30(6):869-877. 2016 [PMID: 27815474] (Western Blot, Rat) Western Blot Rat
Sun , Hualin, Gong , Yanpei, Qiu , Jiaying, Chen , Yanfei, Ding , Fei, Zhao , Qing TRAF6 inhibition rescues dexamethasone-induced muscle atrophy. Int J Mol Sci, 2014;15(6):11126-41. 2014 [PMID: 24955790] (Western Blot, Mouse) Western Blot Mouse
Akkad , Hazem, Corpeno , Rebeca, Larsson , Lars Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE, 2014;9(4):e92622. 2014 [PMID: 24705179] (Western Blot, Rat) Western Blot Rat
Files D, Xiao K, Zhang T, Liu C, Qian J, Zhao W, Morris P, Delbono O, Feng X The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury. PLoS ONE, 2014;9(1):e87587. 2014 [PMID: 24498144] (Western Blot, Mouse) Western Blot Mouse
Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab, 2014;306(7):E723-39. 2014 [PMID: 24425758] (Western Blot, Mouse) Western Blot Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol TRIM63
Uniprot