MuRF1/TRIM63 Antibody [Unconjugated]

Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41‑44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1/TRIM63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MuRF1/TRIM63 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MuRF1/TRIM63
  Met1-Gly325
  Accession # Q969Q1
• Specificity: Detects human, mouse, and rat MuRF1/TRIM63 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM63
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MuRF1/TRIM63 Antibody [Unconjugated]

• EC 6.3.2.-
• IRF
• IRFMURF2
• Iris RING finger protein
• MuRF1
• MuRF-1
• muscle specific ring finger protein 2
• Muscle-specific RING finger protein 1
• RING finger protein 28FLJ32380
• RNF28
• RNF28E3 ubiquitin-protein ligase TRIM63
• SMRZ
• SMRZMURF-1
• Striated muscle RING zinc finger protein
• TRIM63
• tripartite motif containing 63
• tripartite motif-containing 63
• Tripartite motif-containing protein 63

Background

TRIM63 (Tripartite motif-containing protein 63; also MURF-1, SMRZ and RING finger protein 28) is a 41 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is a striated muscle protein that is found in both cytoplasm and nucleus. TRIM63 has multiple finctions, among which are the inhibition of PKC epsilon -mediated cardiomyocyte hypertrophy and the maintenance of skeletal muscle M-line integrity. Human TRIM63 is 353 amino acids (aa) in length. It contains one RING finger domain (aa 23‑82), a B-Box type zinc-finger region (aa 117‑159), a coiled-coil region (aa 207‑269) and a C-terminal COS domain. Isoforms of TRIM63 show one potential alternate start site at Met14, a deletion of aa 105‑132 and a 21 aa substitution for aa 326‑353. Over aa 1‑325, human TRIM63 exhibits 93% aa identity with mouse TRIM63.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MuRF1/TRIM63 Antibody (AF5366)(12)

Publications using AF5366

• DK Suh, WY Lee, WJ Yeo, BS Kyung, KW Jung, HK Seo, YS Lee, DW Suh A Novel Muscle Atrophy Mechanism: Myocyte Degeneration Due to Intracellular Iron Deprivation Cells, 2022-09-13;11(18):. 2022-09-13 [PMID: 36139428] (Mouse)
• DG Campelj, CA Timpani, AC Petersen, A Hayes, CA Goodman, E Rybalka The Paradoxical Effect of PARP Inhibitor BGP-15 on Irinotecan-Induced Cachexia and Skeletal Muscle Dysfunction Cancers, 2020-12-17;12(12):. 2020-12-17 [PMID: 33348673] (Western Blot, Mouse)
• M Noguchi, T Kitakaze, Y Kobayashi, K Mukai, N Harada, R Yamaji &beta-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice Nutrients, 2020-07-22;12(8):. 2020-07-22 [PMID: 32708051] (Western Blot, Mouse)
• P Xia, J Chen, Y Liu, M Fletcher, BC Jensen, Z Cheng Doxorubicin induces cardiomyocyte apoptosis and atrophy through cyclin-dependent kinase 2-mediated activation of forkhead box O1 J. Biol. Chem., 2020-02-19;0(0):. 2020-02-19 [PMID: 32075913] (Western Blot, Mouse, Rat)
• L Zhou, Y Huang, H Xie, X Mei Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels Mol Med Rep, 2017-12-19;0(0):. 2017-12-19 [PMID: 29257347] (Western Blot, Rat)
• H Shimizu, AD Langenbach, J Huang, K Wang, G Otto, R Geisler, Y Wang, JN Chen The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes Elife, 2017-08-19;6(0):. 2017-08-19 [PMID: 28826496] (Western Blot, Zebrafish)
• Apichart Suksamrarn Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy In Vivo, 2016-11-12;30(6):869-877. 2016-11-12 [PMID: 27815474] (Western Blot, Rat)
• Sun , Hualin, Gong , Yanpei, Qiu , Jiaying, Chen , Yanfei, Ding , Fei, Zhao , Qing TRAF6 inhibition rescues dexamethasone-induced muscle atrophy. Int J Mol Sci, 2014-06-20;15(6):11126-41. 2014-06-20 [PMID: 24955790] (Western Blot, Mouse)
• Akkad , Hazem, Corpeno , Rebeca, Larsson , Lars Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE, 2014-04-04;9(4):e92622. 2014-04-04 [PMID: 24705179] (Western Blot, Rat)
• Files D, Xiao K, Zhang T, Liu C, Qian J, Zhao W, Morris P, Delbono O, Feng X The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury. PLoS ONE, 2014-01-31;9(1):e87587. 2014-01-31 [PMID: 24498144] (Western Blot, Mouse)
• Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab, 2014-01-14;306(7):E723-39. 2014-01-14 [PMID: 24425758] (Western Blot, Mouse)
• Wheatley S, El-Kadi S, Suryawan A, Boutry C, Orellana R, Nguyen H, Davis S, Davis T Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of beta-hydroxy-beta-methylbutyrate. Am J Physiol Endocrinol Metab, 2013-11-05;306(1):E91-9. 2013-11-05 [PMID: 24192287] (Western Blot, Porcine)

Bioinformatics

• Gene Symbol: TRIM63
• Uniprot:
  • Q969Q1(Human)