Integrin beta 4/CD104 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cell line HaCaT shows localization to nucleus, plasma membrane & cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human skin shows moderate to strong membranous positivity in keratinocytes.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human endometrium shows moderate to strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cervix, uterine shows moderate to strong membranous positivity in squamous epithelial cells.

Analysis using NBP2-38297 (A) shows similar pattern to independent antibody NBP2-38298 (B).

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Integrin beta 4/CD104 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: MKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRS
• Predicted Species: Mouse (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGB4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Integrin beta 4/CD104 Protein (NBP2-38297PEP)

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34971784) .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Integrin beta 4/CD104 Antibody - BSA Free

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (NBP2-38297)(2)

Publications using NBP2-38297

• Lu Z, Zhong A, Liu H et al. Dissecting the genetic and microenvironmental factors of gastric tumorigenesis in mice Cell reports 2022-10-18 [PMID: 36261019] (ICC/IF, Mouse)
• Fischer NG, Kobe AC, Dai J et al. Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices Acta biomaterialia 2021-12-29 [PMID: 34971784] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • P16144()