Integrin beta 3/CD61 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Integrin beta 3/ CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin beta 3/CD61 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin beta 3/CD61 at approximately 116 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin beta 3/CD61 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2266). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 3/CD61 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61
  Gly27-Asp718
  Accession # P05106
• Specificity: Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 1% cross-reactivity with rhIntegrin beta 1 and rmIntegrin beta 4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 3/CD61 Antibody [Unconjugated]

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 3/CD61 Antibody (AF2266)(3)

Publications using AF2266

• Tabebordbar M, Lagerborg KA, Stanton A et al. Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species Cell 9/16/2021 [PMID: 34506722]
• WM Dickerson, R Yu, HU Westergren, J Paraskos, P Schatz, A Tigerstrom, A Ekman, J Sánchez, J Cheng, L Li, EY Chan Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood PLoS ONE, 2021-08-26;16(8):e0256423. 2021-08-26 [PMID: 34437590] (Interferent Testing, Human)
• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays FEBS Open Bio, 2021-02-03;0(0):. 2021-02-03 [PMID: 33345458] (Western Blot, Human)

Reviews for Integrin beta 3/CD61 Antibody (AF2266) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Nick Dordai ICC Human 12/01/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: SUM159
• Species: Human
• Lot: UII0122021

reviewed by: Verified Customer WB Human 10/20/2022

Summary

• Application: Western Blot
• Sample Tested: human MDAMB-231 whole cell lysate,SUM-159PT
• Species: Human
• Lot: UII0122021

Bioinformatics

• Gene Symbol: ITGB3
• Uniprot:
  • P05106()