Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

Images

 
Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).
Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Human peripheral blood with anti-CD18 (MEM-48) APC.
Radioimmunoassay: Integrin beta 2/CD18 Antibody (MEM-48) [NB500-379] - Analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P, IP, RIA, CyTOF-ready
Clone
MEM-48
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free Summary

Immunogen
Leukocytes of a patient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB2
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 2 ug/ml
Application Notes
Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). This antibody is CyTOF ready.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Integrin beta 2/CD18 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Multi-step

Alternate Names for Integrin beta 2/CD18 Antibody (MEM-48) - BSA Free

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Bioinformatics

Gene Symbol ITGB2