Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (60.3) - Chimeric [NBP3-12031] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 60.3 (NBP3-12031) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12031, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, Inhibition Activity, B/N
• Clone: 60.3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free (NBP3-12031) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.
• Specificity: This antibody binds the Integrin beta 2/CD18 component of the beta-2 integrin family which is expressed by leukocytes and neutrophils.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Functional (Inhibition)
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin beta 2/CD18 Antibody (60.3) - Chimeric - Azide and BSA Free

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB2