Integrin beta 1D Antibody (2B1) - BSA Free

Immunocytochemistry/Immunofluorescence: Integrin beta 1D Antibody (2B1) [NBP1-97734] - Negative control: only secondary antibody applied.

Immunocytochemistry/Immunofluorescence: Integrin beta 1D Antibody (2B1) [NBP1-97734] - Frozen sections of porcine myocardium immunostained for integrin Beta 1D using a 1:200 dillution of 2B1

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, ICC/IF, IHC
• Clone: 2B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin beta 1D Antibody (2B1) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: Derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of Integrin beta 1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
• Specificity: This antibody recognizes specifically the cytoplasmic domain of Integrin beta 1D present in cardiac and skeletal muscle.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:200
  • Western Blot 1:100-1:1000
• Application Notes: This antibody is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..

Reactivity Notes

A broad species reactivity is expected based on the conserved nature of the epitope

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1D Antibody (2B1) - BSA Free

• integrin beta-1
• CD29
• FNRB
• GPIIA
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• MDF2
• MSK12
• VLAB
• VLA-BETA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB1