CD90/Thy1 Antibody

Images

 
Western blot shows lysates of human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Porcine/Canine CD90/Thy1 ...read more
CD90/Thy1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at ...read more
Human mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, ...read more
Porcine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # ...read more
Canine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # ...read more
CD90/Thy1 was detected in immersion fixed canine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room ...read more
CD90/Thy1 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room ...read more
CD90/Thy1 was detected in formalin fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 1.7 ...read more

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

CD90/Thy1 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD90/Thy1
Gln20-Ser141
Accession # P04216
Specificity
Detects human CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD90 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
THY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 uL/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2067 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD90/Thy1 Antibody

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90 (also Thy1/Thymus cell antigen 1) is a 25-35 kDa glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed on neurons, ovarian follicular cells, endothelial cells, fibroblasts and circulating CD34+ stem cells, and appears to act as an adhesion molecule. CD90 is known to bind to integrins beta 2, beta 5 and beta 3, the latter often accompanied by additional binding to syndecan-4. In the postnatal nervous system, this inhibits neurite outgrowth while stabilizing newly formed axonal networks. In the vascular system, CD90 mediates the extravasation of leukocytes. And in lung, a CD90: alpha v beta 5 interaction inhibits the extracellular activation of latent TGF-beta. Mature human CD90 is a 111 amino acid (aa) GPI-linked protein (aa 20-130). It contains one V-type Ig-like domain (aa 20-126) with a heparin-binding motif (aa 56-60), and a GPI-anchor amidated cysteine at position 130. The integrin binding site consists of an Arg35LeuAsp37 tripeptide. CD90 apparently forms 50‑60 kDa homodimers and 150 kDa homomultimers. There is one potential isoform variant that contains a 12 aa substitution for aa 1-13. Over aa 20-141, human CD90 shares 64% aa identity with mouse CD90 and 84% or 83% with horse and dog respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1320
Species: Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-77417
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-56244
Species: Hu
Applications: WB, ICC/IF
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
AF1172
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA
NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP2-66200
Species: Hu
NB100-64843
Species: Mu
Applications: Flow, IHC, IHC-Fr, IHC-P

Publications for CD90/Thy1 Antibody (AF2067) (0)

There are no publications for CD90/Thy1 Antibody (AF2067).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for CD90/Thy1 Antibody (AF2067) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2067:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CD90/Thy1 AF2067
Enlarge
reviewed by:
Anonymous
ICC Human 01/07/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedHMSC cells
SpeciesHuman

Blocking

Blocking Details-

Secondary Antibody

Secondary DescriptionFITC-conjugated donkey anti-sheep IgG

Details

Detection Notes-

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD90/Thy1 Antibody (AF2067). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CD90/Thy1 Antibody (AF2067)

Discover related pathways, diseases and genes to CD90/Thy1 Antibody (AF2067). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD90/Thy1 Antibody (AF2067)

Discover more about diseases related to CD90/Thy1 Antibody (AF2067).
 

Pathways for CD90/Thy1 Antibody (AF2067)

View related products by pathway.

PTMs for CD90/Thy1 Antibody (AF2067)

Learn more about PTMs related to CD90/Thy1 Antibody (AF2067).

Blogs on CD90/Thy1.

CD90 (Cluster of differentiation 90)
CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla...  Read full blog post.

How New Oval Stem Cell Marker Antibodies Will Benefit Hepatic Research
A large number of antibody suppliers supply conjugated and non-conjugated marker antibodies, targeted at specific stem cell populations. Until recently the number of oval stem cell markers was extremely limited. However, we at Novus Biologicals have s...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/07/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol THY1
Uniprot