Integrin alpha V beta 3 Antibody (BV3)

Flow Cytometry: Integrin alpha V beta 3 Antibody (BV3) [NBP3-11213] - HUVEC cells were incubated with 2ug/ml NBP3-11213 for 1h at 4C. Black: isotype control mouse IgG1, Red: cells only, Blue: NBP3-11213.

• Reactivity: Hu, Rt, Ch
• Applications: ELISA, Flow, ICC/IF, IHC, IP
• Clone: BV3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Integrin alpha V beta 3 Antibody (BV3) Summary

• Immunogen: The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAV
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: For Flow Cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha V beta 3 Antibody (BV3)

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V beta 3
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• MSK8
• Vitronectin receptor subunit alpha
• VNRADKFZp686A08142

Background

The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of alphabeta heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The beta3 family consist of 2 members: alphaIIbbeta3 and alphavbeta3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin alphavbeta3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin alphavbeta3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of beta3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin alphavbeta3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin alphavbeta3 has been demonstrated in various tumors and activated endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAV