Integrin alpha 6/CD49f Antibody (GoH3) [Biotin]

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Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [Biotin] [NBP1-43633] - Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k Isotype Control Biotin (open histogram) or 0.125 ug of ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
GoH3
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
0.5 mg/ml

Integrin alpha 6/CD49f Antibody (GoH3) [Biotin] Summary

Immunogen
The immunogen for this antibody was CD49f.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ITGA6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This GoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
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Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26254531)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha 6/CD49f Antibody (GoH3) [Biotin]

  • CD49 antigen-like family member F
  • CD49f antigen
  • CD49f
  • DKFZp686J01244
  • FLJ18737
  • Integrin alpha 6
  • integrin alpha-6
  • integrin alpha6B
  • integrin, alpha 6
  • ITGA6
  • ITGA6B
  • Platelet gpl
  • VLA-6

Background

The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ITGA6
Entrez