Integrin alpha 6/CD49f Antibody (GoH3)

Images

 
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - taining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (NBP1-43400) (open histogram) or 0.25 ug of ...read more
Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the Biotin conjugate of NBP1-43400. Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k Isotype Control Biotin ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
GoH3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Integrin alpha 6/CD49f Antibody (GoH3) Summary

Immunogen
The immunogen for this antibody was CD49f.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ITGA6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This GoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Publications
Read Publications using
NBP1-43400 in the following applications:

  • 2 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26254531)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha 6/CD49f Antibody (GoH3)

  • CD49 antigen-like family member F
  • CD49f antigen
  • CD49f
  • DKFZp686J01244
  • FLJ18737
  • Integrin alpha 6
  • integrin alpha-6
  • integrin alpha6B
  • integrin, alpha 6
  • ITGA6
  • ITGA6B
  • Platelet gpl
  • VLA-6

Background

The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 6/CD49f Antibody (NBP1-43400)(3)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: FLOW.


Filter By Application
FLOW
(2)
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Filter By Species
Bovine
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-43400 Applications Species
Almaraz RT, Tian Y, Bhattarcharya R et al. Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis Mol Cell Proteomics 2012 Mar 28 [PMID: 22457533]
Baratta M, Volpe MG, Nucera D et al. Differential expression of living mammary epithelial cell subpopulations in milk during lactation in dairy cows J Dairy Sci (FLOW, Bovine)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB100-77938).
FLOW Bovine
Borena BM, Bussche L, Getachew Y. Antibiotics for Mastitis Negatively Affect in vitro Cultures of Bovine Mammary Epithelial Stem/Progenitor Cells. European Journal of Biological Sciences. 2015 (FLOW, Bovine) FLOW Bovine

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43400AF405
Alexa Fluor 488 Labeled NBP1-43400AF488
Alexa Fluor 647 Labeled NBP1-43400AF647
Alexa Fluor 700 Labeled NBP1-43400AF700
Allophycocyanin Labeled NBP1-43400APC
Biotin Labeled NBP1-43633
DyLight 350 Labeled NBP1-43400UV
DyLight 405 Labeled NBP1-43400V
DyLight 488 Labeled NBP1-43400G
DyLight 680 Labeled NBP1-43400FR
DyLight 755 Labeled NBP1-43400IR
PE Labeled NBP1-43400PE
PerCP Labeled NBP1-43400PCP

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Bioinformatics

Gene Symbol ITGA6
Entrez