Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Analysis using the Biotin conjugate of NBP1-43400. Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k Isotype Control Biotin (black) or 0.125 ug of Anti-Human/Mouse CD49f Integrin a6 Biotin (purple) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free [NBP2-80815] - Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (NBP1-43400) (open histogram) or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE. Cells in the lymphocyte gate were used for analysis. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Bv
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: GoH3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free Summary

• Immunogen: The immunogen for this antibody was CD49f.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This GoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26254531)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 6/CD49f Antibody (GoH3) - Azide and BSA Free

• CD49 antigen-like family member F
• CD49f antigen
• CD49f
• DKFZp686J01244
• FLJ18737
• Integrin alpha 6
• integrin alpha-6
• integrin alpha6B
• integrin, alpha 6
• ITGA6
• ITGA6B
• Platelet gpl
• VLA-6

Background

The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA6