Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated]


HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), more
Integrin alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233) at 25 µg/mL for 3 hours at room more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready, ICC/IF

Order Details

Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated] Summary

Additional Information
Clone HAS3 was used by HLDA to establish CD designation
Human keratinocytes
Detects human Integrin alpha 2/CD49b.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 15-30 ug/mL
  • Immunoprecipitation
Read Publications using
MAB1233 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated]

  • alpha-2 subunit
  • CD49b antigen
  • CD49b
  • Integrin alpha 2
  • integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
  • ITGA2
  • VLA 2
  • VLA-2 alpha


Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 2/CD49b Antibody (MAB1233)(6)

We have publications tested in 2 confirmed species: Human, Feline.

We have publications tested in 3 applications: Flow Cytometry, Functional Assay, Neutralization.

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Functional Assay
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Showing Publications 1 - 6 of 6.
Publications using MAB1233 Applications Species
Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen. J Biol Chem, 2014-06-23;289(31):21591-604. 2014-06-23 [PMID: 24958723] (Human) Human
Londrigan S, Graham K, Takada Y, Halasz P, Coulson B Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit. J Virol, 2003-09-01;77(17):9486-501. 2003-09-01 [PMID: 12915563] (Neutralization, Human) Neutralization Human
Joling P, Broekhuizen R, de Weger R, Rottier P, Egberink H Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies. Vet Immunol Immunopathol, 1996-09-01;53(1):115-27. 1996-09-01 [PMID: 8941974] (Functional Assay, Feline) Functional Assay Feline
Lisignoli G, Monaco M, Facchini A, Toneguzzi S, Cattini L, Hilbert D, Lavaroni S, Belvedere O, Degrassi A In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation. Eur J Immunol, 1996-01-01;26(1):17-27. 1996-01-01 [PMID: 8566062] (Flow Cytometry, Human) Flow Cytometry Human
Kim I, Uchiyama H, Chauhan D, Anderson K Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines. Br J Haematol, 1994-07-01;87(3):483-93. 1994-07-01 [PMID: 7993788] (Flow Cytometry, Human) Flow Cytometry Human
Kamata T, Takada Y Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner. J Biol Chem, 1994-10-21;269(42):26006-10. 1994-10-21 [PMID: 7523399] (Neutralization, Human) Neutralization Human

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Gene Symbol ITGA2