Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated]

Images

 
Integrin alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233) at 25 µg/mL for 3 hours at room ...read more
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready, ICC/IF
Clone
HAS3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated] Summary

Additional Information
Clone HAS3 was used by HLDA to establish CD designation
Immunogen
Human keratinocytes
Specificity
Detects human Integrin alpha 2/CD49b.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ITGA2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation
  • CyTOF-ready
  • Immunocytochemistry 15-30 ug/mL
Control
Integrin alpha 2/CD49b Lysate (NBP2-64684)
Publications
Read Publications using
MAB1233 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated]

  • alpha-2 subunit
  • CD49b antigen
  • CD49b
  • Integrin alpha 2
  • integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
  • ITGA2
  • VLA 2
  • VLA-2 alpha

Background

Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88928
Species: Hu
Applications: IHC, IHC-P
NB200-541
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF2009
Species: Hu
Applications: IHC, ICC
NBP2-67605
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-32244
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-1598
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NBP1-83790
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF
NBP1-47974
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
MAB6758
Species: Mu
Applications: Flow, CyTOF-ready, ICC
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
MAB1233
Species: Hu
Applications: Flow, IP, CyTOF-ready, ICC/IF

Publications for Integrin alpha 2/CD49b Antibody (MAB1233)(6)

We have publications tested in 2 confirmed species: Human, Feline.

We have publications tested in 3 applications: Flow, Functional Assay, Neut.


Filter By Application
Flow
(2)
Functional Assay
(1)
Neut
(2)
All Applications
Filter By Species
Human
(5)
Feline
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB1233 Applications Species
Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen. J Biol Chem, 2014;289(31):21591-604. 2014 [PMID: 24958723] (Human) Human
Londrigan S, Graham K, Takada Y, Halasz P, Coulson B Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit. J Virol, 0;77(17):9486-501. 0 [PMID: 12915563] (Neut,, Human) Neut Human
Joling P, Broekhuizen R, de Weger R, Rottier P, Egberink H Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies. Vet Immunol Immunopathol, 0;53(1):115-27. 0 [PMID: 8941974] (Functional Assay,, Feline) Functional Assay Feline
Lisignoli G, Monaco M, Facchini A, Toneguzzi S, Cattini L, Hilbert D, Lavaroni S, Belvedere O, Degrassi A In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation. Eur J Immunol, 0;26(1):17-27. 0 [PMID: 8566062] (Flow,, Human) Flow Human
Kim I, Uchiyama H, Chauhan D, Anderson K Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines. Br J Haematol, 0;87(3):483-93. 0 [PMID: 7993788] (Flow,, Human) Flow Human
Kamata T, Takada Y Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner. J Biol Chem, 0;269(42):26006-10. 0 [PMID: 7523399] (Neut,, Human) Neut Human

Reviews for Integrin alpha 2/CD49b Antibody (MAB1233) (0)

There are no reviews for Integrin alpha 2/CD49b Antibody (MAB1233). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin alpha 2/CD49b Antibody (MAB1233) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Integrin alpha 2/CD49b Antibody (MAB1233)

Discover related pathways, diseases and genes to Integrin alpha 2/CD49b Antibody (MAB1233). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Integrin alpha 2/CD49b Antibody (MAB1233)

Discover more about diseases related to Integrin alpha 2/CD49b Antibody (MAB1233).
 

Pathways for Integrin alpha 2/CD49b Antibody (MAB1233)

View related products by pathway.

PTMs for Integrin alpha 2/CD49b Antibody (MAB1233)

Learn more about PTMs related to Integrin alpha 2/CD49b Antibody (MAB1233).

Blogs on Integrin alpha 2/CD49b

There are no specific blogs for Integrin alpha 2/CD49b, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol ITGA2