IL18R1 Antibody (1M2M4)

Western Blot: IL18R1 Antibody (1M2M4) [NBP3-33300] - Western blot analysis of Recombinant Human IL-18R1/CD218a Protein, using IL18R1/IL-18 R alpha Rabbit mAb at1:1600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 10ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 1M2M4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IL18R1 Antibody (1M2M4) Summary

• Description: Novus Biologicals Rabbit IL18R1 Antibody (1M2M4) (NBP3-33300) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 141-320 of human IL18R1 (NP_003846.1).
  
  Sequence:
  ENSYYQTLVNSTSLYKNCKKLLLENNKNPTIKKNAEFEDQGYYSCVHFLHHNGKLFNITKTFNITIVEDRSNIVPVLLGPKLNHVAVELGKNVRLNCSALLNEEDVIYWMFGEENGSDPNIHEEKEMRIMTPEGKWHASKVLRIENIGESNLNVLYNCTVASTGGTDTKSFILVRKADMA
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL18R1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for IL18R1 Antibody (1M2M4)

• CD218 antigen-like family member A
• CD218a antigen
• CD218a
• CDw218a
• cytokine receptor
• IL-18R1
• IL-18R-1
• IL1R-rp
• IL1RRPIL1 receptor-related protein
• IL-1RrpIL18RA
• interleukin 18 receptor 1
• interleukin-18 receptor 1

Background

IL-18 receptor is composed of an alpha and a beta subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor alpha chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. The H44 antibody is specific for IL-18 receptor alpha chain and has been reported to block IL-18 binding to the receptor and to be useful for the identification of IL-18 receptor by flow cytometry and IHC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18R1