IL-1Rrp2/IL-1R6 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1Rrp2/IL-1R6 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑1 Rrp2/IL‑1 R6
  Asp22-Arg338 (Ala158Val)
  Accession # Q9ERS7
• Specificity: Detects mouse IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs, less than 60% with recombinant rat IL-1 Rrp2 and recombinant human IL-1 Rrp2 is observed and less than 5% cross‑reactivity with recombinant mouse (rm) IL-1 R4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il1rl2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1Rrp2/IL-1R6 Antibody [Unconjugated]

• IL-1 R6
• IL-1 Rrp2
• IL1RL2
• IL-1RL2
• IL1Rrp2
• IL-1Rrp2
• IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2
• interleukin 1 receptor-like 2
• interleukin-1 receptor-like 2

Background

IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL‑1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).

1. Dunne, A. and L.A. O’Neill, (2003) Sci. STKE Feb 25 (171):re3.
2. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
3. Lovenberg, T.S. et al. (1996) J. Neuroimmunol. 70:113.
4. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1Rrp2/IL-1R6 Antibody (AF2354)(5)

Publications using AF2354

• Li, D;Qu, J;Pei, Y;Rao, Y;Zhang, Y;Chen, Y;Sun, Y; IL-36R deletion mitigates cigarette smoke-induced airway inflammation and skeletal muscle dysfunction International immunopharmacology 2025-08-06 [PMID: 40773893] (Immunohistochemistry, Western Blot, Immunocytochemistry, Mouse)
• Sachin Shukla, Won Kyung Cho, Elsayed Elbasiony, Rohan Bir Singh, Sharad K. Mittal, Sunil K. Chauhan Non‐immune and immune functions of interleukin‐36 gamma suppress epithelial repair at the ocular surface The FASEB Journal 2/6/2023 [PMID: 35781326]
• J El-Awaisi, DP Kavanagh, MR Rink, CJ Weston, NE Drury, N Kalia Targeting IL-36 improves age-related coronary microcirculatory dysfunction and attenuates myocardial ischaemia-reperfusion injury in mice JCI Insight, 2022-03-08;0(0):. 2022-03-08 [PMID: 35113814] (Flow Cytometry, IHC, Mouse)
• Bozoyan L, Dumas A, Patenaude A, Vallieres L Interleukin-36gamma is expressed by neutrophils and can activate microglia, but has no role in experimental autoimmune encephalomyelitis. J Neuroinflammation, 2015-09-17;12(0):173. 9/17/2015 [PMID: 26377915]
• Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury. Sci Rep, 2015-02-17;5(0):8521. 2015-02-17 [PMID: 25687687] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Il1rl2
• Uniprot:
  • Q9ERS7()