IL-7 Antibody

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Recombinant Mouse IL‑7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap), Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price

IL-7 Antibody Summary

Immunogen
E. coli-derived recombinant mouse IL-7
Glu26-Ile154
Accession # Q544C8
Specificity
Detects mouse IL-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross‑reactivity with recombinant human IL-7 is observed. Neutralizes the biological activity of recombinant mouse IL-7. It will not neutralize the biological activity of recombinant human IL-7.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL7
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.2- 0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse IL-7 Antibody (Catalog # AF407)
ELISA Detection: Mouse IL-7 Biotinylated Antibody (Catalog # BAF407)
Standard:Recombinant Mouse IL-7 (Catalog # 407-ML)
Publications
Read Publications using
AF407 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7 Antibody

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4 + and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma  chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-7 Antibody (AF407)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: ELISA Development, ICC, In vivo, Neut, WB.


Filter By Application
ELISA Development
(1)
ICC
(1)
In vivo
(1)
Neut
(2)
WB
(1)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF407 Applications Species
Shalapour S, Deiser K, Sercan O, Tuckermann J, Minnich K, Willimsky G, Blankenstein T, Hammerling GJ, Arnold B, Schuler T Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo. Eur. J. Immunol., 2010;40(9):2391-400. 2010 [PMID: 20690180] (ICC,, Mouse) ICC Mouse
Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M BCL6 is critical for the development of a diverse primary B cell repertoire. J. Exp. Med., 2010;207(6):1209-21. 2010 [PMID: 20498019] (Neut,, Mouse) Neut Mouse
Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway. J. Immunol., 2009;183(10):6135-44. 2009 [PMID: 19841165] (In vivo,, Mouse) In vivo Mouse
Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Blood, 2007;109(9):3706-12. 2007 [PMID: 17227831] (Neut,, Mouse) Neut Mouse
Lai L, Zeff RA, Goldschneider I A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells. Blood, 2005;107(5):1776-84. 2005 [PMID: 16304052] (WB,, Mouse) WB Mouse
Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J. Immunol., 2005;175(11):7325-31. 2005 [PMID: 16301638] (ELISA Development,, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol IL7
Entrez
Uniprot