IL-4R alpha Antibody (R001) - Azide and BSA Free

Neutralization: IL-4R alpha Antibody (R001) [NBP2-89470] - Recombinant Human IL4 stimulates proliferation in the TF-1 human erythroleukemia cell line. Proliferation elicited by Recombinant Human IL4 (0.2 ng/mL) is neutralized by increasing concentrations of Human IL4R Monoclonal Antibody The IC50 is typically 5-10 ng/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: R001
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IL-4R alpha Antibody (R001) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human IL-4R alpha (Accession#: NP_000409.1; Met1-His232)
• Specificity: No cross-reactivity in ELISA with:
  
  Mouse IL4R
  Rat IL4R
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL4R
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Neutralization
• Application Notes: Neutralization: the neutralization activity of this neutralizing antiboedy is measured by its ability to neutralize IL4 induced proliferation in the human erythroleukemia cell line (TF-1). The Neutralization titer (IC50) is typically 5-50 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL4.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in Histidine and Arginine buffer, 120mM NaCl, 0.02% Tween 80, pH6.0
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for IL-4R alpha Antibody (R001) - Azide and BSA Free

• CD124 antigen
• CD124
• IL-4 R alpha
• IL-4 receptor subunit alpha
• IL4R alpha
• IL-4R alpha
• IL-4R subunit alpha
• IL4R
• IL-4Ra
• IL4RACD124
• IL-4R-alpha
• interleukin 4 receptor
• interleukin-4 receptor alpha chain
• interleukin-4 receptor subunit alpha

Background

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of na ve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL4R