Human IgE Isotype Control

• Reactivity: Hu
• Applications: Flow, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Human
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Human IgE Isotype Control Summary

• Description: This Human IgE immunoglobulin is useful as an isotype control for Human IgE antibody.
• Isotype: IgE
• Clonality: Polyclonal
• Host: Human
• Gene: IGHE
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow (Intracellular) 1:10 - 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl, pH 8.
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Human IgE Isotype Control

• IgE
• immunoglobulin heavy constant epsilon

Background

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHE