IL-4 Antibody (BVD4-1D11) - BSA Free

Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in mouse primary myoblasts at day 6 (white arrows indicate myoblasts, grey arrows indicate myotubes). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in undifferentiated ESCs. Scale bar: 20 um. Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.

Western Blot: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 followed by F(ab')2 RABBIT ANTI RAT IgG:HRP.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, IHC
• Clone: BVD4-1D11
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-4 Antibody (BVD4-1D11) - BSA Free Summary

• Description: Novus Biologicals Rat IL-4 Antibody (BVD4-1D11) - BSA Free (NB100-64798) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-IL-4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant Mouse IL-4
• Specificity: This is specific for mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells. Clone BVD4-1D11 is reported to neutralise the effects of mouse IL-4 (3).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml-5 ug/ml
  • Flow Cytometry 1:10-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1 ug/ml-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-4 Antibody (BVD4-1D11) - BSA Free

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of native CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4 Antibody (NB100-64798)(2)

Publications using NB100-64798

• Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-11-14 [PMID: 36373718] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Swierczek-Lasek B, Neska J et al. Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion. Int J Mol Sci 2019-08-13 [PMID: 31412558] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: IL4
• Entrez:
  • 16189(Mouse)
  • 16189(Mouse)
  • 287287(Rat)
• Uniprot:
  • P07750()
  • P07750()