IL-3R alpha/CD123 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-3R alpha/CD123 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑3 R alpha /CD123
  Ser17-Lys331
  Accession # P26952
• Specificity: Detects mouse IL‑3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL3RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3R alpha/CD123 Antibody [Unconjugated]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and the beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta . AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha , also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

1. Itoh, N. et al. (1990) Science 247:324.
2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
4. Schrader, J.W. (2001) Cytokine Reference, Oppenheim , J.J. and M. Feldmann, eds, Academic Press, New York, p. 1899.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-3R alpha/CD123 Antibody (AF983)(1)

Publication using AF983

• Chenxi Wei, Huaxiao Wen, Langyue Yuan, Cliona M. McHale, Hui Li, Kun Wang et al. Formaldehyde induces toxicity in mouse bone marrow and hematopoietic stem/progenitor cells and enhances benzene-induced adverse effects Archives of Toxicology 2017-02-01 [PMID: 27339418] (Western Blot, Mouse)

Review for IL-3R alpha/CD123 Antibody (AF983) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 06/04/2019

Summary

• Application: Simple Western
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: FMB011121

Bioinformatics

• Gene Symbol: IL3RA
• Uniprot:
  • P26952()