IL-3R alpha/CD123 Antibody [Unconjugated]

Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-3R alpha/CD123 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF841) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-3R alpha/CD123 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Characteristics of sEV isolated from AML patients’ plasma at diagnosis: (A) transmission electron microscopy (TEM) of isolated sEV, (B) size and concentration of sEV as determined by NanoSight, (C) Western blots show that sEV carry endocytic markers (Alix, CD9, and TSG101) but not cytoplasmic proteins such as Grp94 or Calnexin, and (D) Western blots show the presence of LAAs in the isolated sEV. LAAs are differentially expressed in each patient’s sEV. Each lane was loaded with 10 μg of sEV protein. TSG101 is a marker that confirms the endocytic origin of sEV. NC: sEV isolated from healthy donors’ plasma. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38137457), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-3R alpha/CD123 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123
• Specificity: Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 2% cross‑reactivity with recombinant human (rh) IL-2 sR beta , rhIL-2 sR gamma , and rhIL-6 sR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL3RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3R alpha/CD123 Antibody [Unconjugated]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.

1. Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA