IL-3R alpha/CD123 Antibody (18N6B12)

Western Blot: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis of CD123 in the A) presence and B) absence of equal amounts antibody and immunizing peptide in human SKNSH cell lysate using CD123 antibody at 10 ug/ml.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27225. Staining of CD123 in human buffy coat using anti-CD123 antibody. Image from verified customer review.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Cell surface analysis using CD123 antibody. BDCM cells were probed using 1 ug of CD123 antibody (red) or 1 ug of isotype control (green). This IL-3 R alpha/CD123 antibody and a mouse IgG-PE secondary antibody were used for this test.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27225. Staining of CD123 in human buffy coat using anti-CD123 antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 18N6B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IL-3R alpha/CD123 Antibody (18N6B12) Summary

• Description: Novus Biologicals Mouse IL-3R alpha/CD123 Antibody (18N6B12) (NBP2-27225) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 250-300 of human CD123 was used as the immunogen for this antibody.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ug/0.5x10^6cells
  • Western Blot 10ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IL-3R alpha/CD123 Antibody (18N6B12)

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

CD123 (Cluster of Differentiation 123), also known as Interleukin-3 receptor subunit alpha, is an Interleukin 3 specific subunit of a heterodimeric cytokine receptor. CD123 is a low affinity receptor to interleukin-3 and is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3, colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5. The CD123 beta subunit is activated by the ligand binding, and is required for the biological activities of IL-3. A member of type I cytokine receptor family and type 5 subfamily, CD123 functions as a signaling cytokine known to stimulate cell proliferation, differentiation and survival. The gene for this protein maps within the X-Y pseudoautosomal region (PAR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA
• Uniprot:
  • P26951()