IL-38/IL-1F10 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-38/IL-1F10 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human IL‑38/IL‑1F10
  Cys2-Trp152
  Accession # Q8WWZ1
• Specificity: Detects human IL‑38/IL‑1F10 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha , rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1F9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1F10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody [Biotin]

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Background

Human Il-38/IL-1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-38/IL-1F10 Antibody (BAF2427)(2)

Publications using BAF2427

• D Talabot-Ay, L Mermoud, J Borowczyk, J Drukala, M Wolnicki, A Modarressi, WH Boehncke, N Brembilla, G Palmer Interleukin-38 interacts with destrin/actin-depolymerizing factor in human keratinocytes PLoS ONE, 2019-11-26;14(11):e0225782. 2019-11-26 [PMID: 31770407] (Western Blot, Human)
• van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist. Proc. Natl. Acad. Sci. U.S.A., 2012-02-06;109(8):3001-5. 2012-02-06 [PMID: 22315422] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL1F10
• Uniprot:
  • Q8WWZ1()