Home » IL-38/IL-1F10 » IL-38/IL-1F10 Antibodies » IL-38/IL-1F10 Antibody [Unconjugated]

IL-38/IL-1F10 Antibody [Unconjugated]

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Recombinant Human IL‑38/IL‑1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑38/IL‑1F10 Monoclonal Antibody(Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑38/IL‑1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human IL‑38/IL‑1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑38/IL‑1F10 Monoclonal Antibody(Catalog # MAB24271) coated on a Clear Polystyrene Microplate ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Biotin
BAF2427
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Catalog# & Formulation Size Price
Carrier Free
AF2427-SP
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IL-38/IL-1F10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL‑38/IL‑1F10
Cys2-Trp152
Accession # Q8WWZ1
Specificity
Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha , rhIL-1 beta , rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1 F9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1F10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF2427 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody [Unconjugated]

  • FIL1- theta
  • FKSG75
  • IL1F10
  • IL-1HY2
  • IL38
  • IL-38
  • interleukin 1 family, member 10 (theta)
  • UNQ6119/PRO20041

Background

Human IL‑38/IL‑1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-38/IL-1F10 Antibody (AF2427)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol IL1F10
Uniprot