IL-38/IL-1F10 Antibody [Unconjugated]

Recombinant Human IL‑38/IL‑1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑38/IL‑1F10 Monoclonal Antibody(Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑38/IL‑1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-38/IL-1F10 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑38/IL‑1F10
  Cys2-Trp152
  Accession # Q8WWZ1
• Specificity: Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1 F9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1F10

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody [Unconjugated]

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Background

Human IL‑38/IL‑1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-38/IL-1F10 Antibody (AF2427)(1)

Publication using AF2427

• D Talabot-Ay, L Mermoud, J Borowczyk, J Drukala, M Wolnicki, A Modarressi, WH Boehncke, N Brembilla, G Palmer Interleukin-38 interacts with destrin/actin-depolymerizing factor in human keratinocytes PLoS ONE, 2019;14(11):e0225782. 2019 [PMID: 31770407] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL1F10
• Uniprot:
  • Q8WWZ1()