IL-23A/IL-23 P19 Antibody

Western blot shows lysates of human mature dendritic cells (LPS activated), CLL-1390 canine leukocytic round cell neoplasia cell line, and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Canine IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6250) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Ca
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-23A/IL-23 P19 Antibody Summary

• Immunogen: E. coli-derived recombinant canine IL-23 p19
  Arg23-Pro193
  Accession # XP_538231
• Specificity: Detects human and canine IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant feline IL-23 p19 is observed and approximately 40% cross-reactivity with recombinant mouse IL-23 p19 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IL23A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23A/IL-23 P19 Antibody

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

IL-23 p19 (Interleukin 23 p19; also IL-23 alpha) is an 18-20 kDa (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-gamma production by CD4⁺, CD8⁺ and NK cells. Based on human, mature canine IL-23 p19 is 171 amino acids (aa) in length. It is a alpha -helical molecule that utilizes Cys77 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature canine IL-23 p19 (aa 23-193) shares 88% aa identity with human IL-23 p19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL23A
• Uniprot:
  • XP_538231()