IL-23A/IL-23 P19 Antibody [Unconjugated]

Images

 
Western blot shows lysates of a CHO cell line transfected with human IL-23. A PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716) ...read more
In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402‑ML), Recombinant Human IL‑23 (Catalog # 1290-IL) stim­ulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-23A/IL-23 P19 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL‑23 p19
Arg20-Pro189
Accession # AAG37232
Specificity
Detects human IL‑23 p19 in direct ELISA and Western blot. In direct ELISA, approximately 50% cross-reactivity with recombinant canine
IL-23p19, less then 25% cross-reactivity with recombinant feline IL-23p19, less than 5% cross-reactivity with recombinant mouse IL-23p19, recombinant human IL-12p40, and recombinant rat IL-23p40 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL23A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 0.2-0.8 ug/mL
Publications
Read Publications using
AF1716 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23A/IL-23 P19 Antibody [Unconjugated]

  • IL-23 p19/IL-12 p40
  • IL23
  • IL-23
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL-23p19/IL-12p40
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-23A/IL-23 P19 Antibody (AF1716)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development, IHC-Fr, Neutralization.


Filter By Application
ELISA Development
(1)
IHC-Fr
(1)
Neutralization
(5)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1716 Applications Species
Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. J Immunol, 2012;189(12):5590-601. 2012 [PMID: 23152566] (Neutralization, Human) Neutralization Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neutralization, Human) Neutralization Human
Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. J. Immunol., 2011;186(7):4175-82. 2011 [PMID: 21368235] (Neutralization, Human) Neutralization Human
Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis. Br. J. Dermatol., 2011;164(6):1228-34. 2011 [PMID: 21299543] (IHC-Fr, Human) IHC-Fr Human
Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Eur. J. Immunol., 2009;39(5):1221-30. 2009 [PMID: 19350551] (Neutralization, Human) Neutralization Human
de Paus RA, van de Wetering D, Van Dissel JT IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants. Mol. Immunol., 2008;45(15):3889-95. 2008 [PMID: 18675459] (Neutralization, Human) Neutralization Human
Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC. Cytokine, 2006;35(5):235-46. 2006 [PMID: 17052915] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol IL23A
Uniprot