IL-21R Antibody (152512)

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 152512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-21R Antibody (152512) Summary

• Additional Information: Clone 152512 was used by HLDA to establish CD designation
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R
  Cys20-Pro236
  Accession # Q9HBE5
• Specificity: Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL21R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21R Antibody (152512)

• CD360 antigen
• CD360
• IL-21 R
• IL-21 receptor
• IL21R
• IL-21R
• interleukin 21 receptor
• interleukin-21 receptor
• MGC10967
• NILR
• Novel interleukin receptor

Background

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta , IL-4 R alpha , and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma  chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

1. Parrish-Novak, et al. (2000) Nature 408:57.
2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao, H. et al. (2001) J. Immunol. 167:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-21R Antibody (MAB9911)(3)

Publications using MAB9911

• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human)
• Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P New B-cell CD molecules. Immunol. Lett., 2010-10-07;134(2):104-12. 2010-10-07 [PMID: 20933010] (Flow Cytometry, Human)
• Ansen S, Butler MO, Berezovskaya A, Murray AP, Stevenson K, Nadler LM, Hirano N Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21. Clin. Cancer Res., 2008-10-01;14(19):6125-36. 2008-10-01 [PMID: 18829491] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL21R
• Uniprot:
  • Q9HBE5()