IL-21R Antibody (152512) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-21 R PE-conjugated Monoclonal Antibody (Catalog # FAB9911P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 152512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-21R Antibody (152512) [Phycoerythrin] Summary

• Additional Information: Clone 152512 was used by HLDA to establish CD designation
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 R
  Cys20-Pro236
  Accession # Q9HBE5
• Specificity: Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL21R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21R Antibody (152512) [Phycoerythrin]

• CD360 antigen
• CD360
• IL-21 R
• IL-21 receptor
• IL21R
• IL-21R
• interleukin 21 receptor
• interleukin-21 receptor
• MGC10967
• NILR
• Novel interleukin receptor

Background

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta , IL-4 R alpha , and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma  chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

1. Parrish-Novak, et al. (2000) Nature 408:57.
2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao, H. et al. (2001) J. Immunol. 167:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-21R Antibody (FAB9911P)(6)

Publications using FAB9911P

• Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia. J. Immunol., 2011-03-25;186(9):5435-42. 2011-03-25 [PMID: 21441456] (Flow Cytometry, Human)
• Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription. Immunology, 2011-01-07;132(4):540-8. 2011-01-07 [PMID: 21214545] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)
• de Totero D, Capaia M, Fabbi M, Croce M, Meazza R, Cutrona G, Zupo S, Loiacono F, Truini M, Ferrarini M, Ferrini S Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells. Exp. Hematol., 2010-03-01;38(5):373-83. 2010-03-01 [PMID: 20193734] (Flow Cytometry, Human)
• Lee WW, Kang SW, Choi J Regulating human Th17 cells via differential expression of IL-1 receptor. Blood, 2009-11-12;115(3):530-40. 2009-11-12 [PMID: 19965648] (Flow Cytometry, Human)
• Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M Effect of IL-21 on NK cells derived from different umbilical cord blood populations. Int Immunol, 2005-11-15;18(1):49-58. 2005-11-15 [PMID: 16291655] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL21R
• Uniprot:
  • Q9HBE5()