IL-21 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-21 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
  Pro25-Ser146
  Accession # Q9ES17
• Specificity: Detects mouse IL-21 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL‑15, and recombinant human IL‑21 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL21
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse IL-21 Antibody (Catalog # AF594)
  ELISA Detection: Mouse IL-21 Biotinylated Antibody (Catalog # BAF594)
  Standard: Recombinant Mouse IL-21 (Catalog # 594-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha . Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

1. Parrish-Novak, et al. (2000) Nature 408:57.
2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao, H. et al. (2001) J. Immunol. 167:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL21