Recombinant Mouse IL-21R Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IL-21R Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 75-500 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-21 R protein
Mouse IL-21 R Subunit
(Cys20 - Pro236)
Accession # Q9JHX3
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Cys20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il21r
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-80 kDa, reducing conditions
Publications
Read Publications using
596-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-21R Fc Chimera Protein, CF

  • CD360 antigen
  • CD360
  • IL-21 R
  • IL-21 receptor
  • IL21R
  • IL-21R
  • interleukin 21 receptor
  • interleukin-21 receptor
  • MGC10967
  • NILR
  • Novel interleukin receptor

Background

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma  chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Konforte, D. et al. (2009) J. Immunol. 182:1791.
  3. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Asao, H. et al. (2001) J. Immunol. 167:1.
  7. Habib, T. et al. (2002) Biochemistry 41:8725.
  8. Ozaki, K. et al. (2002) Science 298:1630.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. King, I.L. et al. (2010) J. Immunol. 185:6138.
  11. Kasaian, M.T. et al. (2002) Immunity 16:559.
  12. Frohlich, A. et al. (2009 Science 324:1576.
  13. Tortola, L. et al. (2010) Blood 116:5200.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.

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Publications for IL-21R (596-MR)(28)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, Flow Cytometry, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
Cell Culture
(1)
Flow Cytometry
(17)
In Vivo
(3)
All Applications
Filter By Species
Mouse
(28)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 596-MR Applications Species
L Wang, E Shen, L Luo, H Rabe, Q Wang, J Yin, X Yang, W Liu, JM Sido, H Nakagawa, L Ao, HJ Kim, H Cantor, JW Leavenwort Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor Cell Rep, 2019;29(7):1848-1861.e6. 2019 [PMID: 31722202] (Cell Culture, Mouse) Cell Culture Mouse
MD Powell, KA Read, BK Sreekumar, DM Jones, KJ Oestreich IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population Sci Rep, 2019;9(1):13991. 2019 [PMID: 31570752] (Flow Cytometry, Mouse) Flow Cytometry Mouse
JS Park, SM Kim, J Choi, KA Jung, SH Hwang, S Yang, SK Kwok, ML Cho, SH Park Interleukin-21-mediated suppression of the Pax3-Id3 pathway exacerbates the development of Sj�gren&#039;s syndrome via follicular helper T cells Cytokine, 2019;125(0):154834. 2019 [PMID: 31491724] (In Vivo, Mouse) In Vivo Mouse
D Cortes-Sel, A Ready, L Gibbs, B Rajwa, KC Fairfax IL-4 Promotes stromal cell expansion and Is critical for development Of A Type-2, but not a type 1 immune response Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30575951] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Q Cheng, J Liu, Y Pei, Y Zhang, D Zhou, W Pan, J Zhang Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection PLoS Pathog., 2018;14(11):e1007440. 2018 [PMID: 30462731] (Flow Cytometry, Mouse) Flow Cytometry Mouse
CT Johndrow, MF Goldberg, AJ Johnson, TW Ng, S Kunnath-Ve, G Lauvau, DH Kaplan, GH Gossel, UD Kadolsky, AJ Yates, J Chan, WR Jacobs, SA Porcelli Suppression of Th1 Priming by TLR2 Agonists during Cutaneous Immunization Is Mediated by Recruited CCR2+ Monocytes J. Immunol., 2018;0(0):. 2018 [PMID: 30455402] (Flow Cytometry, Mouse) Flow Cytometry Mouse
KM Valentine, D Davini, TJ Lawrence, GN Mullins, M Manansala, M Al-Kuhlani, JM Pinney, JK Davis, AE Beaudin, SS Sindi, DM Gravano, KK Hoyer CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease J. Immunol., 2018;0(0):. 2018 [PMID: 29743314] (Bioassay, Mouse) Bioassay Mouse
JY Choi, A Seth, M Kashgarian, S Terrillon, E Fung, L Huang, LC Wang, J Craft Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus J. Immunol, 2017;0(0):. 2017 [PMID: 28219887] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Antigen-presenting cell-derived IL-6 restricts the expression of GATA3 and IL-4 by follicular helper T cells J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27474166] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Jang E, Cho W, Oh Y, Cho M, Kim J, Paik D, Youn J Splenic Long-Lived Plasma Cells Promote the Development of Follicular Helper T Cells during Autoimmune Responses. J Immunol, 2016;196(3):1026-35. 2016 [PMID: 26729802] (Bioassay, Mouse) Bioassay Mouse
Show All 28 Publications.

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Bioinformatics

Gene Symbol Il21r
Uniprot