Recombinant Mouse IL-21R Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-21R Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 75-500 ng/mL.
Mouse myeloma cell line, NS0-derived mouse IL-21 R protein
Mouse IL-21 R Subunit
(Cys20 - Pro236)
Accession # Q9JHX3
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
51.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
70-80 kDa, reducing conditions
Read Publications using
596-MR in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-21R Fc Chimera Protein, CF

  • CD360 antigen
  • CD360
  • IL-21 R
  • IL-21 receptor
  • IL21R
  • IL-21R
  • interleukin 21 receptor
  • interleukin-21 receptor
  • MGC10967
  • NILR
  • Novel interleukin receptor


IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma  chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Konforte, D. et al. (2009) J. Immunol. 182:1791.
  3. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Asao, H. et al. (2001) J. Immunol. 167:1.
  7. Habib, T. et al. (2002) Biochemistry 41:8725.
  8. Ozaki, K. et al. (2002) Science 298:1630.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. King, I.L. et al. (2010) J. Immunol. 185:6138.
  11. Kasaian, M.T. et al. (2002) Immunity 16:559.
  12. Frohlich, A. et al. (2009 Science 324:1576.
  13. Tortola, L. et al. (2010) Blood 116:5200.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.

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Publications for IL-21R (596-MR)(30)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, Flow Cytometry, ICC, In Vivo.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 596-MR Applications Species
D Cortés-Sel, L Gibbs, A Ready, HA Ekiz, R O'Connell, B Rajwa, KC Fairfax Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion PloS Pathogens, 2021;17(2):e1009260. 2021 [PMID: 33524040] (Bioassay, Mouse) Bioassay Mouse
LE Latham, DJ Wikenheise, JS Stumhofer ICOS signaling promotes a secondary humoral response after re-challenge with Plasmodium�chabaudi chabaudi AS PLoS Pathog., 2020;16(4):e1008527. 2020 [PMID: 32348365] (ICC, Mouse) ICC Mouse
L Wang, E Shen, L Luo, H Rabe, Q Wang, J Yin, X Yang, W Liu, JM Sido, H Nakagawa, L Ao, HJ Kim, H Cantor, JW Leavenwort Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor Cell Rep, 2019;29(7):1848-1861.e6. 2019 [PMID: 31722202] (Cell Culture, Mouse) Cell Culture Mouse
MD Powell, KA Read, BK Sreekumar, DM Jones, KJ Oestreich IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population Sci Rep, 2019;9(1):13991. 2019 [PMID: 31570752] (Flow Cytometry, Mouse) Flow Cytometry Mouse
JS Park, SM Kim, J Choi, KA Jung, SH Hwang, S Yang, SK Kwok, ML Cho, SH Park Interleukin-21-mediated suppression of the Pax3-Id3 pathway exacerbates the development of Sj�gren&#039;s syndrome via follicular helper T cells Cytokine, 2019;125(0):154834. 2019 [PMID: 31491724] (In Vivo, Mouse) In Vivo Mouse
D Cortes-Sel, A Ready, L Gibbs, B Rajwa, KC Fairfax IL-4 Promotes stromal cell expansion and Is critical for development Of A Type-2, but not a type 1 immune response Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30575951] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Q Cheng, J Liu, Y Pei, Y Zhang, D Zhou, W Pan, J Zhang Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection PLoS Pathog., 2018;14(11):e1007440. 2018 [PMID: 30462731] (Flow Cytometry, Mouse) Flow Cytometry Mouse
CT Johndrow, MF Goldberg, AJ Johnson, TW Ng, S Kunnath-Ve, G Lauvau, DH Kaplan, GH Gossel, UD Kadolsky, AJ Yates, J Chan, WR Jacobs, SA Porcelli Suppression of Th1 Priming by TLR2 Agonists during Cutaneous Immunization Is Mediated by Recruited CCR2+ Monocytes J. Immunol., 2018;0(0):. 2018 [PMID: 30455402] (Flow Cytometry, Mouse) Flow Cytometry Mouse
KM Valentine, D Davini, TJ Lawrence, GN Mullins, M Manansala, M Al-Kuhlani, JM Pinney, JK Davis, AE Beaudin, SS Sindi, DM Gravano, KK Hoyer CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease J. Immunol., 2018;0(0):. 2018 [PMID: 29743314] (Bioassay, Mouse) Bioassay Mouse
JY Choi, A Seth, M Kashgarian, S Terrillon, E Fung, L Huang, LC Wang, J Craft Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus J. Immunol, 2017;0(0):. 2017 [PMID: 28219887] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Show All 30 Publications.

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Gene Symbol Il21r