IL-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human tonsil. Image from verified customer review.

Flow Cytometry: IL-2 Antibody [NBP2-16948] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: IL2 antibody dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.

Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human gastric cancer, using IL2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Untreated (-) and treated (+) Jurkat E6.1 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL2 antibody (NBP2-16948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, RM
• Applications: WB, Flow, Func, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IL-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-2 Antibody - BSA Free (NBP2-16948) is a polyclonal antibody validated for use in IHC, WB and Flow. Anti-IL-2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IL-2. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • In-situ Hybridization Reported in scientific literature (PMID:35083300)
  • Western Blot 1:500-1:3000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IL-2 Antibody - BSA Free

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IL-2 Antibody (NBP2-16948)(4)

Publications using NBP2-16948

• Zhao RJ, Lee DG, Park CS et al. Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System Evidence-based complementary and alternative medicine : eCAM 2022-10-22 [PMID: 36317105] (IF/IHC, Rat)
• Li Q, Zhang Z, Chen S et al. miR-190a-5p Partially Represses the Abnormal Electrical Activity of SCN3B in Cardiac Arrhythmias by Downregulation of IL-2 Frontiers in cardiovascular medicine 2022-01-10 [PMID: 35083300] (ISH, Human)
• Derricott H, Jones RL, Greenwood SL et al. Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth. The American Journal of Pathology. 2016-04-18 [PMID: 26851347] (IHC-P, Mouse)
• Arafa RS, Abdel Haie OM, El-Azab DS et al. Significant hepatic expression of IL-2 and IL-8 in biliary atresia compared with other neonatal cholestatic disorders. Cytokine. 2016-03-01 [PMID: 26765485] (IHC-P, Human)

Review for IL-2 Antibody (NBP2-16948) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Hayley Derricott IHC-P Human 09/03/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human tonsil
• Species: Human
• Lot: 40702

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P60568()