IL-2 Antibody

Images

 
Western Blot: IL-2 Antibody [NBP2-16948] - Sample (30 ug of whole cell lysate) A: Raji 15% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human tonsil. Image from verified customer review.
Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Immunohistochemical analysis of paraffin-embedded Gastric cancer, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.91 mg/ml

Order Details

IL-2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IL2. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-16948 in the following applications:

Publications
Read Publications using
NBP2-16948 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-2 Antibody

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-2 Antibody (NBP2-16948)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Review for IL-2 Antibody (NBP2-16948) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP2-16948:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IL-2 NBP2-16948
Enlarge
reviewed by:
Hayley Derricott
IHC-P Human 09/03/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot40702

Blocking

Blocking DetailsNon-immune block (swine serum, human serum and TBS-T 0.1%) at room temp for 30 mins.

Primary Anitbody

Dilution Ratio5µg/mL, diluted in non-immune block, incubated at 37 degrees for one hour

Secondary Antibody

Secondary DescriptionBiotinylated swine anti-rabbit IgG
Secondary Concentration4µg/mL

Details

Detection NotesDetection with DAB, 2-3 minutes
Fixation DetailsFixed in 4% NBF, paraffin wax embedded, heat mediated antigen retrieval in citrate buffer (20 mins)
Wash Description2 x 5 min wash with TBS, 2 x 5 min wash with TBS-T 0.6%

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-2 Antibody (NBP2-16948) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IL-2 Antibody (NBP2-16948)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Hayley Derricott
09/03/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IL2
Entrez
Uniprot