IL-17RA/IL-17R Antibody (JE35-89)

Western Blot: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Western blot analysis of IL-17RA/IL-17R on different lysates with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: HL-60 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 96 kDa Observed band size: 160 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32467) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Immunocytochemistry analysis of HeLa cells labeling IL-17RA/IL-17R with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: JE35-89
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IL-17RA/IL-17R Antibody (JE35-89) Summary

• Description: Novus Biologicals Rabbit IL-17RA/IL-17R Antibody (JE35-89) (NBP3-32467) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human IL-17RA/IL-17R aa 801-850 / 866. (Uniprot: Q96F46)
• Localization: Cell membrane, Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL17RA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-17RA/IL-17R Antibody (JE35-89)

• CD217 antigen
• CD217
• Cdw217
• CDw217interleukin 17 receptor
• hIL-17R
• IL-17 R
• IL-17 RA
• IL-17 receptor A
• IL17RA
• IL-17RA
• IL-17RAMGC10262
• IL17Rinterleukin-17 receptor A
• interleukin 17 receptor A

Background

IL17RA is a gene that codes for a widely expressed protein that functions as a receptor for IL17A (a proinflammatory cytokine secreted by T-lymphocytes) with low affinity, which suggests that additional components are necessary to induce signaling and has a length of 866 amino acids and a weight of approximately 96 kDa. Current studies are being done on several diseases and disorders related to this gene including rheumatoid arthritis, cat eye syndrome, candidiasis, pituitary adenoma, bronchiolitis, cystic fibrosis, Sjogren's syndrome, keratitis, myocarditis, hepatitis B, liver disease, gingivitis, and osteoarthritis. IL17RA has also been shown to have interactions with IL17F, IL17RE, UBC, and TRAF3IP2 in pathways such as the IL-21 signaling, NF-kappaB signaling, and cytokine production pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL17RA