IL-17RD/SEF Antibody

K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2275, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

IL‑17 RD/SEF was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2275) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glomerulli and endothelial cells in capillaries in connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RD/SEF Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑17 RD/SEF
  Ala27-Arg299
  Accession # AAM77571
• Specificity: Detects human IL-17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) IL-17 RD is observed and less than 10% cross-reactivity with recombinant human (rh) IL-17 RC, rmIL-17B R, and rhIL-17 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17RD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RD/SEF Antibody

• FLJ35755
• IL-17 RD
• IL17RD
• IL-17RD
• IL-17RDIL-17 receptor D
• IL17Rhom
• IL17RLM
• IL17RLMDKFZp434N1928
• interleukin 17 receptor D
• interleukin-17 receptor D
• Interleukin-17 receptor-like protein
• MGC133309
• Sef homolog
• SEF
• SEFhSef

Background

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1-5). The gene for this protein belongs to a synexpression group originally identified in zebrafish where SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). By alternate splicing, two transcript variants, potentially encoding three protein isoforms, exist. One is a full-length long form, one a shortened form that uses an alternate start site, and one an alternate splice form that removes the classic signal sequence (1-4). These isoforms have different expression patterns, subcellular localization, and function. The membrane-bound long form of human IL-17RD is synthesized as a 739 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 420 aa cytoplastic domain. The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). Unlike the alternate splice form of IL-17 RD that has a restricted pattern of expression, the full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
5. Lin, W. et al. (2002) Mech. Dev. 113:163.
6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
8. Torii, S. et al. (2004) Dev. Cell 7:33.
9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RD/SEF Antibody (AF2275)(2)

Publications using AF2275

• J Pekow, K Meckel, U Dougherty, Y Huang, X Chen, A Almoghrabi, R Mustafi, F Ayaloglu-B, Z Deng, HI Haider, J Hart, DT Rubin, JH Kwon, M Bissonnett miR-193a-3p is a key tumor suppressor in ulcerative colitis-associated colon cancer and promotes carcinogenesis through up-regulation of IL17RD Clin. Cancer Res., 2017-06-09;0(0):. 2017-06-09 [PMID: 28600480] (IHC, Western Blot, Human)
• HUI ZHANG, QIUFEN GUO, XIA WANG, CHONG WANG, XINGBO ZHAO, MINGJIANG LI Aberrant expression of hSef and Sprouty4 in endometrial adenocarcinoma Oncology Letters 2016-01-01 [PMID: 26870165]

Review for IL-17RD/SEF Antibody (AF2275) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22975329
• Species: Human

Bioinformatics

• Gene Symbol: IL17RD
• Uniprot:
  • AAM77571()