IL-17RD/SEF Antibody

Images

 
IL‑17 RD/SEF was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody ...read more
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2275, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IL-17RD/SEF Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RD/SEF
Ala27-Arg299
Accession # AAM77571
Specificity
Detects human IL‑17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) IL-17 RD is observed and less than 10% cross-reactivity with recombinant human (rh) IL-17 RC, rmIL-17B R, and rhIL-17 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17RD
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2275 in the following applications:

Publications
Read Publication using
AF2275 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RD/SEF Antibody

  • FLJ35755
  • IL-17 RD
  • IL17RD
  • IL-17RD
  • IL-17RDIL-17 receptor D
  • IL17Rhom
  • IL17RLM
  • IL17RLMDKFZp434N1928
  • interleukin 17 receptor D
  • interleukin-17 receptor D
  • Interleukin-17 receptor-like protein
  • MGC133309
  • Sef homolog
  • SEF
  • SEFhSef

Background

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1-5). The gene for this protein belongs to a synexpression group originally identified in zebrafish where SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). By alternate splicing, two transcript variants, potentially encoding three protein isoforms, exist. One is a full-length long form, one a shortened form that uses an alternate start site, and one an alternate splice form that removes the classic signal sequence (1-4). These isoforms have different expression patterns, subcellular localization, and function. The membrane-bound long form of human IL-17RD is synthesized as a 739 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 420 aa cytoplastic domain. The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). Unlike the alternate splice form of IL-17 RD that has a restricted pattern of expression, the full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

  1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
  2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
  3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
  4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
  5. Lin, W. et al. (2002) Mech. Dev. 113:163.
  6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
  7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
  8. Torii, S. et al. (2004) Dev. Cell 7:33.
  9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
DY421
Species: Mu
Applications: ELISA
233-FB/CF
Species: Hu
Applications: BA
NBP1-47974
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB177
Species: Hu
Applications: CyTOF-ready, Flow, KO, Neut, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
423-F8
Species: Hu, Mu
Applications: BA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47833
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF2340
Species: Hu
Applications: CyTOF-ready, Flow, IP, WB
AF3749
Species: Hu
Applications: IHC, WB
NBP2-33278
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-91148
Species: Hu
Applications: IHC, IHC-P
NB100-56740
Species: Hu, Mu
Applications: IHC, IHC-P, IP, Simple Western, WB
H00006301-M01
Species: Hu, Mu, Ze
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, KD, WB
NBP2-20262
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF2275
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for IL-17RD/SEF Antibody (AF2275)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for IL-17RD/SEF Antibody (AF2275) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2275:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22975329
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-17RD/SEF Antibody (AF2275) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-17RD/SEF Products

Bioinformatics Tool for IL-17RD/SEF Antibody (AF2275)

Discover related pathways, diseases and genes to IL-17RD/SEF Antibody (AF2275). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-17RD/SEF Antibody (AF2275)

Discover more about diseases related to IL-17RD/SEF Antibody (AF2275).
 

Pathways for IL-17RD/SEF Antibody (AF2275)

View related products by pathway.

PTMs for IL-17RD/SEF Antibody (AF2275)

Learn more about PTMs related to IL-17RD/SEF Antibody (AF2275).

Blogs on IL-17RD/SEF

There are no specific blogs for IL-17RD/SEF, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous -
01/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL17RD
Uniprot