Recombinant Mouse IL-17/IL-17A Protein

Product Discontinued

Recombinant Mouse IL-17/IL-17A Protein Summary

• Description: Recombinant bioactive protein containing 266 amino acids for Mouse IL-17/IL-17A
  
  

  Source:E. coli
  Amino Acid Sequence:(Q62386)

• Details of Functionality: The mouse IL-17 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent secretion of IL-6 by NIH 3T3 cells was found to be 1.0 -10.0 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL17A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from solution containing no additives.
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to 0.1 - 1.0 ug/ul. The solution can be diluted into other aqueous buffers andstored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Mouse IL-17/IL-17A Protein

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Recombinant murine IL-17 is a homodimeric, non-glycosylated polypeptide containing 266 amino acids and has a molecular weight of 30 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

FAQs for IL-17/IL-17A Recombinant Protein (NBP1-99822). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A