Rat IL-17/IL-17A ELISA Kit (Colorimetric)

ELISA: IL-17/IL-17A ELISA Kit [HRP] [NBP1-92705] - Standard curve

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 1.0 pg/mL

Rat IL-17/IL-17A ELISA Kit (Colorimetric) Summary

• Description: The rat IL-17A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-17A.
• Specificity: Crossreactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-17A positive sample. There was no crossreactivity detected, notably not with rat IFN gamma, rat TNF alpha, rat IL-1 alpha, rat IL-4, rat MCP-1, rat GM-CSF.
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 1.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.6%
• Intra-Assay: CV% < 8.5%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL17A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction, as well as increased survival locally. IL-17A and IL-17F production by T lymphocytes is regulated by IL-23 independent of T cell receptor activation. The Th1 and Th2 cell classification has until recently provided the framework for understanding CD4+ T cell biology and the interplay between innate and adaptive immunity. The Th17 lineage produces interleukin 17, which is highly proinflammatory and induces severe autoimmunity. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-17/IL-17A ELISA Kit (NBP1-92705)(1)

Publication using NBP1-92705

• McColl, ER;Henderson, JT;Piquette-Miller, M; Dysregulation of Amino Acid Transporters in a Rat Model of TLR7-Mediated Maternal Immune Activation Pharmaceutics 2023-07-01 [PMID: 37514044] (ELISA)

FAQs for IL-17/IL-17A ELISA Kit (NBP1-92705). (Showing 1 - 3 of 3 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
3. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL17A