IL-17/IL-17A Antibody [Unconjugated]

Images

 
IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 ...read more
Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen ...read more
Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 ...read more
Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) ...read more
Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

IL-17/IL-17A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human IL-17F is observed and less than 5% cross-reactivity with recombinant mouse IL‑17 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 3 ug/100 ug cell lysate
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.02-0.12 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF-317-NA in the following applications:

Publications
Read Publications using
AF-317-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF-317-NA
Species: Hu
Applications: WB, IHC, IP, B/N, CyTOF-ready, Flow, ICC/IF

Publications for IL-17/IL-17A Antibody (AF-317-NA)(45)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 10 applications: Cell Culture, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Tissue Culture, Western Blot.


Filter By Application
Cell Culture
(1)
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(14)
IHC-Fr
(1)
IHC-P
(15)
Neutralization
(8)
Tissue Culture
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(42)
Mouse
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using AF-317-NA Applications Species
ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31948839] (Tissue Culture, Human) Tissue Culture Human
L Fania, M Morelli, C Scarponi, L Mercurio, F Scopelliti, C Cattani, GL Scaglione, T Tonanzi, MA Pilla, G Pagnanelli, C Mazzanti, G Girolomoni, A Cavani, S Madonna, C Albanesi Paradoxical psoriasis induced by Tnf-alpha blockade shows immunological features typical of the early phase of psoriasis development J Pathol Clin Res, 2019;0(0):. 2019 [PMID: 31577850] (IHC, Human) IHC Human
R Khalmurato, HW Shin, DW Kim, JW Park Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2 EBioMedicine, 2019;0(0):. 2019 [PMID: 31331833] (Cell Culture, Mouse) Cell Culture Mouse
M Boxberg, L Leising, K Steiger, M Jesinghaus, A Alkhamas, M Mielke, N Pfarr, C Götz, KD Wolff, W Weichert, A Kolk Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma J. Immunol., 2018;0(0):. 2018 [PMID: 30530592]
DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis Sci Rep, 2017;7(1):13283. 2017 [PMID: 29038590] (IHC, Human) IHC Human
J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation Sci Rep, 2017;7(1):10310. 2017 [PMID: 28871165] (Flow Cytometry, Canine) Flow Cytometry Canine
C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments PLoS ONE, 2017;12(8):e0183415. 2017 [PMID: 28829805] (IHC-P, Human) IHC-P Human
CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells Oncoimmunology, 2016;5(12):e1242543. 2016 [PMID: 28439450] (IHC-P, Human) IHC-P Human
Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways PLoS ONE, 2016;11(8):e0161433. 2016 [PMID: 27552197] (IHC-P, Human) IHC-P Human
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues Lab Invest, 2016;0(0):. 2016 [PMID: 27348627] (IHC-P, Human) IHC-P Human
Show All 45 Publications.

Reviews for IL-17/IL-17A Antibody (AF-317-NA) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.

Reviews using AF-317-NA:
Filter by Applications
ICC
(2)
IHC-P
(2)
All Applications
Filter by Species
Human
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry IL-17/IL-17A AF-317-NA
Enlarge
5
reviewed by:
Manuel Flores
ICC Human 03/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedFFPE,FFPE human liver
SpeciesHuman

Comments

CommentsMultiplex immnunofluorescence labelling IL-17A, CD4, and FoxP3 on FFPE section of human liver biopsy. IL-17A primary Ab is the AF-317-NA from Novus Biologicals. Secondary Ab is A568. FFPE sections were dewaxed with xylene and rehydrated with ethanol solutions (decreasing %). Antigen retrieval was performed with sodium citrate pH6 in a pressure cooker. IL-17A signal is specific, and the staining pattern is what you would expect for a molecule that is produced in the cytoplasm and secreted.
  4
reviewed by:
Anonymous
IHC-P Human 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20426778
SpeciesHuman
  4
reviewed by:
Anonymous
IHC-P Other 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20357258
SpeciesOther
  4
reviewed by:
Anonymous
ICC Human 01/07/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 21307293
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21307293

Details

Detection NotesSee PMID 21307293

Comments

CommentsPublished: See PMID 21307293Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-17/IL-17A Antibody (AF-317-NA). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF317

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Bioinformatics Tool for IL-17/IL-17A Antibody (AF-317-NA)

Discover related pathways, diseases and genes to IL-17/IL-17A Antibody (AF-317-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-17/IL-17A Antibody (AF-317-NA)

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Manuel Flores
03/24/2017
Application: ICC
Species: Human

 
Anonymous
01/07/2015
Application: IHC-P
Species: Human

 
Anonymous
01/07/2015
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol IL17A
Uniprot