IL-17/IL-17A Antibody [Unconjugated]

IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

IL-17A mRNA (red) and IL17A protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human Crohn’s Disease Intestine. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IL-17A (Catalog # 310931) and goat anti-human IL-17/IL-17A polyclonal antibody (R&D Systems Catalog # AF-317-NA) at 1ug/mL. Tissue was stained using RNAscope® 2.5 HD Detection Kit-RED (Catalog # 322360) and RNAscope® 2.5 LS Green Accessory Pack (Catalog # 322550). Tissue was counterstained with 50% hematoxylin (blue).

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca 2+ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Recombinant Human IL-17 (317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02‑0.12 µg/mL.

Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of IL‐17A+ cells, a reduction of dermal IFN‐ gamma + cells and an increase of IL‐22+ or IL‐36 gamma + cells, when compared with psoriatic skin lesions. LS and NLS skin of the same psoriatic patient (n = 3) was analyzed. Graphs show the mean of number of positive cells + SD per three sections. One out of three representative stainings is shown. *p < 0.01, **p < 0.05, versus classical psoriasis. Scale bars, 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31577850), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar = 50 µm). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL-17 upregulates FAP expression by activating the p-STAT3 signaling pathway.A Protein levels of FAP, pSTAT3 (Tyr705), pSTAT3 (Ser727), and STAT3 after 20 ng/ml IL-17a stimulation or PBS treatment. B Protein levels of FAP, IL-17, ACTA2, and VIMENTIN in IL-17-OE cells, FAP-OE cells, and the corresponding negative control cell lines. C Protein levels of pSTAT3 (Tyr705), pSTAT3 (Ser727), STAT3, pP65, and P65 in IL-17-OE cells, FAP-OE cells, and the corresponding negative control cell lines. D The binding ability of pSTAT3 (Tyr705) and STAT3 to the FAP promoter region in FAP-OE and FAP-NC cells was evaluated by a CUT&RUN assay. E The binding ability of pSTAT3 (Tyr705) and STAT3 to the FAP promoter region in IL-17-OE and IL-17-NC cells was evaluated by a CUT&RUN assay. F The mRNA expression of FAP of LX2 cell line after 24 h treatment of 50 μg/mL Colivelin. G The expression of FAP, STAT3, and p-STAT3 in LX2 cell line after 24 h treatment of 50 μg/mL Colivelin. H The FAP, STAT3, p-STAT3 level in FAP-OE and FAP-NC cell lines after 2 μM HJC0152 treatment. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41420-024-01995-4), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: IHC, IP, B/N, CyTOF-ready, Dual ISH-IHC, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17/IL-17A Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-17
  Ile20-Ala155
  Accession # Q16552
• Specificity: Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17A
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 0.5 - 5 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 3 ug/100 ug cell lysate
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.02-0.12 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody [Unconjugated]

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17/IL-17A Antibody (AF-317-NA)(96)

Publications using AF-317-NA

• Rosenzweig, N;Kleemann, KL;Rust, T;Carpenter, M;Grucci, M;Aronchik, M;Brouwer, N;Valenbreder, I;Cooper-Hohn, J;Iyer, M;Krishnan, RK;Sivanathan, KN;Brandão, W;Yahya, T;Durao, A;Yin, Z;Chadarevian, JP;Properzi, MJ;Nowarski, R;Davtyan, H;Weiner, HL;Blurton-Jones, M;Yang, HS;Eggen, BJL;Sperling, RA;Butovsky, O; Sex-dependent APOE4 neutrophil-microglia interactions drive cognitive impairment in Alzheimer's disease Nature medicine 2024-07-03 [PMID: 38961225] (Immunohistochemistry, Human)
• Tsukalov, I;Sánchez-Cerrillo, I;Rajas, O;Avalos, E;Iturricastillo, G;Esparcia, L;Buzón, MJ;Genescà, M;Scagnetti, C;Popova, O;Martin-Cófreces, N;Calvet-Mirabent, M;Marcos-Jimenez, A;Martínez-Fleta, P;Delgado-Arévalo, C;de Los Santos, I;Muñoz-Calleja, C;Calzada, MJ;González Álvaro, I;Palacios-Calvo, J;Alfranca, A;Ancochea, J;Sánchez-Madrid, F;Martin-Gayo, E; NF?B and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins Nature communications 2024-03-07 [PMID: 38453949] (Immunohistochemistry, Immunocytochemistry, Human)
• Mick J.M. van Eijs, José J.M. Ter Linde, Matthijs J.D. Baars, Mojtaba Amini, Miangela M. Laclé, Eelco C. Brand et al. Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis iScience 2023-10-20 [PMID: 37766980] (Mass Cytometry, Immunohistochemistry, Imaging Mass Cytometry, Human)
• Maria Luigia Carbone, Alessia Capone, Marika Guercio, Sofia Reddel, Domenico Alessandro Silvestris, Daniela Lulli et al. Insight into immune profile associated with vitiligo onset and anti-tumoral response in melanoma patients receiving anti-PD-1 immunotherapy Frontiers in Immunology 2023-08-23 [PMID: 37680638]
• Oyler BL, Valencia-Dávila JA, Moysi E et al. Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches iScience 2023-08-18 [PMID: 37520703]
• Xue Liu, Chenyang Xu, Tianwei Guo, Shenghua Zhan, Qiuying Quan, Mengsi Li et al. Clinical significance of CD155 expression and correlation with cellular components of tumor microenvironment in gastric adenocarcinoma Frontiers in Immunology 2023-06-27 [PMID: 37441080]
• Jennifer S. Chen, Michael J. Murphy, Katelyn Singh, Alice Wang, Ryan D. Chow, Sa Rang Kim et al. IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies JID Innovations 2023-07-01 [PMID: 37205304]
• Simon Bossart, S. Morteza Seyed Jafari, Kristine Heidemeyer, Kexiang Yan, Laurence Feldmeyer, Luca Borradori et al. Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome Frontiers in Medicine 2023-03-15 [PMID: 37007766] (Immunohistochemistry, Human)
• A Schäbitz, C Hillig, M Mubarak, M Jargosch, A Farnoud, E Scala, N Kurzen, AC Pilz, N Bhalla, J Thomas, M Stahle, T Biedermann, CB Schmidt-We, F Theis, N Garzorz-St, K Eyerich, MP Menden, S Eyerich Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases Nature Communications, 2022-12-13;13(1):7729. 2022-12-13 [PMID: 36513651] (IHC, Human)
• Zheng N, Fang J, Xue G et al. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance Cancer cell 8/15/2022 [PMID: 36027915]
• Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindström et al. The immune cell atlas of human neuroblastoma Cell Reports Medicine 2022-06-09 [PMID: 35688160] (Immunohistochemistry, Human)
• G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 2022-01-10;13(1):181. 2022-01-10 [PMID: 35013299] (IHC, Human)
• Daniëlle Krijgsman, Neeraj Sinha, Matthijs J.D. Baars, Stephanie van Dam, Mojtaba Amini, Yvonne Vercoulen MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data STAR Protocols 2022-03-01 [PMID: 34977680] (Immunohistochemistry, Human)
• A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma Biomedicines, 2021-12-16;9(12):. 2021-12-16 [PMID: 34944746] (IHC, Human)
• CF Kelley, I Pollack, R Yacoub, Z Zhu, VE Van Doren, S Gumber, RR Amara, V Fedirko, CS Kraft, TJB de Man, YJ Hu, C Grimsley A, PS Sullivan, RM Bostick Condomless receptive anal intercourse is associated with markers of mucosal inflammation in a cohort of men who have sex with men in Atlanta, Georgia Journal of the International AIDS Society, 2021-12-01;24(12):e25859. 2021-12-01 [PMID: 34911162] (IHC, Human)
• Jing Li, Lisha Cheng, Haoyu Jia, Chun Liu, Siqi Wang, Yun Liu et al. IFN‐ gamma facilitates liver fibrogenesis by CD161+CD4+ T cells through a regenerative IL‐23/IL‐17 axis in chronic hepatitis B virus infection Clinical & Translational Immunology 2021-11-02 [PMID: 34754450] (Neutralization, Human)
• Daniela Alejandra Rios, Paola Cecilia Casciato, María Soledad Caldirola, María Isabel Gaillard, Cecilia Giadans, Beatriz Ameigeiras et al. Chronic Hepatitis C Pathogenesis: Immune Response in the Liver Microenvironment and Peripheral Compartment Frontiers in Cellular and Infection Microbiology 2021-08-03 [PMID: 34414132]
• D Lamb, D De Sousa, K Quast, K Fundel-Cle, JS Erjefält, C Sandén, HJ Hoffmann, M Kästle, R Schmid, K Menden, D Delic ROR&amp;gammat inhibitors block both IL-17 and IL-22 conferring a potential advantage over anti-IL-17 alone to treat severe asthma Respiratory Research, 2021-05-22;22(1):158. 2021-05-22 [PMID: 34022896] (IHC, Human)
• V Cairoli, E De Matteo, D Rios, C Lezama, M Galoppo, P Casciato, E Mullen, C Giadans, G Bertot, MV Preciado, P Valva Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease Scientific Reports, 2021-03-04;11(1):5129. 2021-03-04 [PMID: 33664397] (IHC, Human)
• Michael P. Crawford, Sushmita Sinha, Pranav S. Renavikar, Nicholas Borcherding, Nitin J. Karandikar CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression Proceedings of the National Academy of Sciences 2020-08-11 [PMID: 32719138]
• Katsuya Gunjigake, Jun Kinoshita, Takahisa Yamaguchi, Hiroto Saito, Daisuke Fujimori, Toshihide Horiike et al. Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination Gastric Cancer 2021-01-01 [PMID: 32488650]
• Guido Trentadue, Gursah Kats-Ugurlu, Tjasso Blokzijl, Gilles FH Diercks, Jan Willem Haveman, Klaas Nico Faber et al. Safe and Successful Treatment of Acute Cellular Rejection of an Intestine and Abdominal Wall Transplant With Vedolizumab Transplantation Direct 2020-02-01 [PMID: 32095513]
• ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019-12-27;0(0):. 2019-12-27 [PMID: 31948839] (Tissue Culture, Human)
• L Fania, M Morelli, C Scarponi, L Mercurio, F Scopelliti, C Cattani, GL Scaglione, T Tonanzi, MA Pilla, G Pagnanelli, C Mazzanti, G Girolomoni, A Cavani, S Madonna, C Albanesi Paradoxical psoriasis induced by Tnf-alpha blockade shows immunological features typical of the early phase of psoriasis development J Pathol Clin Res, 2019-10-29;0(0):. 2019-10-29 [PMID: 31577850] (IHC, Human)
• R Khalmurato, HW Shin, DW Kim, JW Park Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2 EBioMedicine, 2019-07-19;0(0):. 2019-07-19 [PMID: 31331833] (Cell Culture, Mouse)
• Na Guo, Ge Shen, Ying Zhang, Ahmed A. Moustafa, Dongxia Ge, Zongbing You Interleukin-17 Promotes Migration and Invasion of Human Cancer Cells Through Upregulation of MTA1 Expression Frontiers in Oncology 2019-06-20 [PMID: 31281798] (Immunohistochemistry, Human)
• Frew John W, Hawkes Jason E, Krueger James G A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Research 6/25/2019 [PMID: 31281635]
• John W. Frew, Jason E. Hawkes, James G. Krueger A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa F1000Research 2018-12-13 [PMID: 30828428]
• M Boxberg, L Leising, K Steiger, M Jesinghaus, A Alkhamas, M Mielke, N Pfarr, C Götz, KD Wolff, W Weichert, A Kolk Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma J. Immunol., 2018-12-10;0(0):. 2018-12-10 [PMID: 30530592] (IHC-P, Human)
• Clarissa R. Teixeira, Claire da S. Santos, Deboraci B. Prates, Rafael T. dos Santos, Théo Araújo-Santos, Sebastião M. de Souza-Neto et al. Lutzomyia longipalpis Saliva Drives Interleukin-17-Induced Neutrophil Recruitment Favoring Leishmania infantum Infection Frontiers in Microbiology 2018-05-08 [PMID: 29867796] (Neutralization, Human)
• Nicola Tamassia, Fabio Arruda-Silva, Federica Calzetti, Silvia Lonardi, Sara Gasperini, Elisa Gardiman et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It Frontiers in Immunology 2018-04-17 [PMID: 29719541]
• Weijie Zhong, Xin Xu, Zhigang Zhu, Li Yang, Hong Du, Zhongjun Xia et al. Increased interleukin-17A levels promote rituximab resistance by suppressing p53 expression and predict an unfavorable prognosis in patients with diffuse large B cell lymphoma International Journal of Oncology 2018-03-05 [PMID: 29512700]
• Y Chen, M Zhong, G Yuan, H Peng Interleukin-17 induces angiogenesis in�vitro via CXCL8 and CCL2 in retinal pigment epithelium Mol Med Rep, 2018-01-18;0(0):. 1/18/2018 [PMID: 29363728]
• DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis Sci Rep, 2017-10-16;7(1):13283. 2017-10-16 [PMID: 29038590] (IHC, Human)
• J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation Sci Rep, 2017-09-04;7(1):10310. 2017-09-04 [PMID: 28871165] (Flow Cytometry, Canine)
• C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments PLoS ONE, 2017-08-22;12(8):e0183415. 2017-08-22 [PMID: 28829805] (IHC-P, Human)
• CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells Oncoimmunology, 2016-10-14;5(12):e1242543. 2016-10-14 [PMID: 28439450] (IHC-P, Human)
• Cheryl Schofield, Saloumeh K Fischer, Michael J Townsend, Sofia Mosesova, Kun Peng, Alvernia Francesca Setiadi et al. Characterization of IL-17AA and IL-17FF in rheumatoid arthritis and multiple sclerosis Bioanalysis 2016-11-01 [PMID: 27620302] (ELISA Detection, Human)
• Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways PLoS ONE, 2016-08-23;11(8):e0161433. 2016-08-23 [PMID: 27552197] (IHC-P, Human)
• Claire Deleage, Alexandra Schuetz, W. Gregory Alvord, Leslie Johnston, Xing-Pei Hao, David R. Morcock et al. Impact of early cART in the gut during acute HIV infection JCI Insight 2016-07-07 [PMID: 27446990]
• Jennifer M. Myers, Leslie T. Cooper, David C. Kem, Stavros Stavrakis, Stanley D. Kosanke, Ethan M. Shevach et al. Cardiac myosin-Th17 responses promote heart failure in human myocarditis JCI Insight 2016-06-16 [PMID: 27366791]
• Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues Lab Invest, 2016-06-27;0(0):. 2016-06-27 [PMID: 27348627] (IHC-P, Human)
• Alexander Gelbard, Nicolas-George Katsantonis, Masanobu Mizuta, Dawn Newcomb, Joseph Rotsinger, Bernard Rousseau et al. Idiopathic subglottic stenosis is associated with activation of the inflammatory IL-17A/IL-23 axis The Laryngoscope 2016-11-01 [PMID: 27296163]
• Serge Camelo, Sophie Lavelette, Xavier Guillonneau, William Raoul, Florian Sennlaub Association of Choroidal Interleukin-17-Producing T Lymphocytes and Macrophages with Geographic Atrophy Ophthalmologica 2016-01-01 [PMID: 27287199]
• Neal L Millar IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy Sci Rep, 2016-06-06;6(0):27149. 2016-06-06 [PMID: 27263531] (IHC, Human)
• Netanya S. Utay, Annelys Roque, J. Katherina Timmer, David R. Morcock, Claire DeLeage, Anoma Somasunderam et al. MRSA Infections in HIV-Infected People Are Associated with Decreased MRSA-Specific Th1 Immunity PLOS Pathogens 2016-04-19 [PMID: 27093273] (Immunohistochemistry, Human)
• Ana Paula Vieira, Maria Ângela Bianconcini Avancini, Carla Pagliari, João Avancini, Alberto José da Silva Sakai-Valente, Alberto José da Silva Sakai-Valente et al. Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells The American Journal of Tropical Medicine and Hygiene 2016-04-06 [PMID: 26903606]
• Simone Punt, Emilie A. C. Dronkers, Marij J. P. Welters, Renske Goedemans, Senada Koljenović, Elisabeth Bloemena et al. A beneficial tumor microenvironment in oropharyngeal squamous cell carcinoma is characterized by a high T cell and low IL-17(+) cell frequency Cancer Immunology, Immunotherapy 2016-04-01 [PMID: 26899388] (Immunohistochemistry, Human)
• Muhammad S. Alam, Matthias M. Gaida, Frank Bergmann, Felix Lasitschka, Thomas Giese, Nathalia A. Giese et al. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression Nature Medicine 2015-11-01 [PMID: 26479921] (Immunohistochemistry, Human)
• Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells? PLoS ONE, 2015-06-12;10(6):e0129426. 2015-06-12 [PMID: 26069967] (IHC, Human)
• Stefan M Brunner, Florian Weber, Jens M Werner, Ayman Agha, Stefan A Farkas, Hans J Schlitt et al. Neuroendocrine tumors of the pancreas: a retrospective single-center analysis using the ENETS TNM-classification and immunohistochemical markers for risk stratification BMC Surgery 2015-12-01 [PMID: 25928025]
• Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer. Mol Cancer, 2015-03-31;14(0):71. 2015-03-31 [PMID: 25889974] (IHC-P, Human)
• Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice. Eur J Immunol, 2015-05-15;45(6):1667-79. 2015-05-15 [PMID: 25820779] (IHC-P, Human)
• Simone Punt, Marjolein E. van Vliet, Vivian M. Spaans, Cornelis D. de Kroon, Gert Jan Fleuren, Arko Gorter et al. FoxP3(+) and IL-17(+) cells are correlated with improved prognosis in cervical adenocarcinoma Cancer Immunology, Immunotherapy 2015-06-01 [PMID: 25795131]
• Yu , Qing, Lou , Xiang-mi, He , Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015-03-13;10(3):e0120855. 2015-03-13 [PMID: 25768730] (IHC-P, Human)
• Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD. Respir Res, 2014-11-27;15(1):145. 2014-11-27 [PMID: 25427574] (IHC-P, Western Blot, Human)
• Liu , Xiaosun, Jin , Hailong, Zhang , Geer, Lin , Xianke, Chen , Chao, Sun , Jianyi, Zhang , Yu, Zhang , Qing, Yu , Jiren Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients. PLoS ONE, 2014-09-08;9(9):e106834. 2014-09-08 [PMID: 25197971] (IHC, Human)
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Reviews for IL-17/IL-17A Antibody (AF-317-NA) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Other.

reviewed by: Manuel Flores ICC Human 03/24/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: FFPE human liver,FFPE
• Species: Human

Comments

• Comments: Multiplex immnunofluorescence labelling IL-17A , CD4 , and FoxP3 on FFPE section of human liver biopsy. IL-17A primary Ab is the AF-317-NA from Novus Biologicals. Secondary Ab is A568. FFPE sections were dewaxed with xylene and rehydrated with ethanol solutions (decreasing %). Antigen retrieval was performed with sodium citrate pH6 in a pressure cooker. IL-17A signal is specific, and the staining pattern is what you would expect for a molecule that is produced in the cytoplasm and secreted.

reviewed by: Verified Customer IHC-P Human 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 20426778
• Species: Human

reviewed by: Verified Customer IHC-P Other 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 20357258
• Species: Other

reviewed by: Verified Customer ICC Human 01/07/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 21307293
• Species: Human

FAQs for IL-17/IL-17A Antibody (AF-317-NA). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q16552()