IL-17/IL-17A Antibody (665909)

Recombinant Canine IL‑17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL‑17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL‑17 Monoclonal Antibody (Catalog # MAB5848). The ND50 is typically 5-30 ng/mL.

• Reactivity: Ca
• Applications: B/N
• Clone: 665909
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-17/IL-17A Antibody (665909) Summary

• Immunogen: E. coli-derived recombinant canine IL-17
  Gly26-Ala155
  Accession # NP_001159350
• Specificity: Detects canine IL-17 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) IL-17A is observed and no cross-reactivity with rhIL-17F or recombinant mouse IL-17A is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 5-30 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (665909)

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot which characterize a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL‑17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8⁺ memory T cells (10-11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL‑17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15).  The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL‑17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha , IL‑1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL‑17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, GRO‑ alpha , and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).

1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
5. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
7. Steinman, L. (2007) Nat. Med. 13:139.
8. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
9. Lockhart, E. et al. (2006) J. Immunol. 177:4662.
10. Ferretti, S. et al. (2003) J. Immunol. 170:2106.
11. Shin, H.C. et al. (1999) Cytokine 11:257.
12. Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430.
13. Zhou, Q. et al. (2005) Infect. Immun. 73:935.
14. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
15. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
16. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
17. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
18. Yao, Z. et al. (1995) Immunity 3:811.
19. Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.
20. Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559.
21. Albanesi, C. et al. (1999) J. Immunol. 162:494.
22. Witowski, J. et al. (2000) J. Immunol. 165:5814.
23. Miyamoto, M. et al. (2003) J. Immunol. 170:4665.
24. Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335.
25. Laan, M. et al. (2001) Br. J. Pharmacol. 133:200.
26. Starnes, T. et al. (2002) J. Immunol. 169:642.
27. Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748.
28. Yao, Z. et al. (1995) J. Immunol. 155:5483.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IL-17/IL-17A Antibody (MAB5848). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • NP_001159350()