IL-17/IL-17A Antibody (50104) [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Mouse IL‑17/IL‑17A (Catalog # 421-ML), Recombinant Human IL‑17/IL‑17A (Catalog # 317-ILB), Recombinant Rat IL‑17/IL‑17A (Catalog # 8410-IL), and ...read more
Recombinant Mouse IL‑17 (Catalog # 421-ML) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
50104
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IL-17/IL-17A Antibody (50104) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Specificity
Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant rat IL-17A and approximately 40% reactivity with recombinant mouse
(rm) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant human IL-17, recombinant canine IL-17, rmIL-17B,
rmIL‑17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL17A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 0.05-0.15 ug/mL
Publications
Read Publications using
MAB421 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (50104) [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17/IL-17A Antibody (MAB421)(84)

We have publications tested in 2 confirmed species: Mouse, Bacteria - Porphyromonas gingivalis.

We have publications tested in 6 applications: Blocking, ELISA Development (Capture), Functional Assay, In vivo, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
ELISA Development (Capture)
(1)
Functional Assay
(1)
In vivo
(1)
Neutralization
(78)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(83)
Bacteria - Porphyromonas gingivalis
(1)
All Species
Showing Publications 1 - 10 of 84. Show All 84 Publications.
Publications using MAB421 Applications Species
S Qiao, H Zhang, X Zha, W Niu, J Liang, G Pang, Y Tang, T Liu, H Zhao, Y Wang, H Bai Endogenous IL-17A mediated neutrophil infiltration by promoting chemokines expression during chlamydial lung infection Microb. Pathog., 2019;0(0):. 2019 [PMID: 30703475] (Neutralization, Mouse) Neutralization Mouse
F Chen, W Wu, L Jin, A Millman, M Palma, DW El-Naccach, KE Lothstein, C Dong, KL Edelblum, WC Gause B Cells Produce the Tissue-Protective Protein RELM? during Helminth Infection, which Inhibits IL-17�Expression and Limits Emphysema Cell Rep, 2018;25(10):2775-2783.e3. 2018 [PMID: 30517865] (Neutralization, Mouse) Neutralization Mouse
AP Gounder, CC Yokoyama, NN Jarjour, TL Bricker, BT Edelson, ACM Boon Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer PLoS Pathog., 2018;14(4):e1007001. 2018 [PMID: 29698474] (Western Blot, Mouse) Western Blot Mouse
BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin J Am Heart Assoc, 2018;7(8):. 2018 [PMID: 29654199] (Neutralization, Mouse) Neutralization Mouse
CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis Cell Death Dis, 2018;9(3):401. 2018 [PMID: 29540697] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis Am. J. Pathol., 2018;0(0):. 2018 [PMID: 29458010] (Neutralization, Mouse) Neutralization Mouse
M Geha, MG Tsokos, RE Bosse, T Sannikova, Y Iwakura, JJ Dalle Lucc, R De Waal Ma, GC Tsokos IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury J. Immunol., 2017;0(0):. 2017 [PMID: 28877988] (Neutralization, Mouse) Neutralization Mouse
JM Garth, KM Reeder, MS Godwin, JJ Mackel, CW Dunaway, JP Blackburn, C Steele IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection J. Immunol., 2017;199(6):2140-2148. 2017 [PMID: 28784844] (Neutralization, Mouse) Neutralization Mouse
RC Davicino, SP Méndez-Hue, RJ Eliçabe, JC Stupirski, I Autenrieth, MS Di Genaro, GA Rabinovich Galectin-1-Driven Tolerogenic Programs Aggravate Yersinia enterocolitica Infection by Repressing Antibacterial Immunity J. Immunol., 2017;0(0):. 2017 [PMID: 28716827] (Neutralization, Mouse) Neutralization Mouse
JM Zou, J Qin, YC Li, Y Wang, D Li, Y Shu, C Luo, SS Wang, G Chi, F Guo, GM Zhang, ZH Feng IL-35 induces N2 phenotype of neutrophils to promote tumor growth Oncotarget, 2017;8(20):33501-33514. 2017 [PMID: 28432279] (Neutralization, Mouse) Neutralization Mouse
Show All 84 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-17/IL-17A Antibody (MAB421). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol IL17A
Uniprot