Human IL-10 ELISA Kit (Colorimetric)


ELISA: IL-10 ELISA Kit [NBP1-89871] - Representative standard curve for human IL-10 ELISA. Human IL-10 was diluted in serial 2-fold steps in Assay Buffer (1x)

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (citrate, heparin)
Standard Curve Range
3.15 - 200 pg/mL
1.0 pg/mL

Order Details

Human IL-10 ELISA Kit (Colorimetric) Summary

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-10 positive serum. There was no crossreactivity detected.
Standard Curve Range
3.15 - 200 pg/mL
1.0 pg/mL
Assay Type
Sandwich ELISA
Spike Recovery
The recovery ranged from 81% to 106% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-10.

Reactivity Notes


Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-10
  2. Amplification Reagent II
  3. Amplification Diluent Concentrate (2x)
  4. Amplification Reagent I*
  5. Adhesive Films
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. * reagent contains ethyl alcohol
  8. Biotin-Conjugate anti-human IL-10 monoclonal antibody
  9. Control, lyophilized
  10. Human IL-10 Standard lyophilized, 400 pg/mL upon reconstitution
  11. Stop Solution (1M Phosphoric acid)
  12. Streptavidin-HRP
  13. Sample Diluent
  14. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  15. Substrate Solution (tetramethyl-benzidine)

Alternate Names for Human IL-10 ELISA Kit (Colorimetric)

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)

Publications for IL-10 ELISA Kit (NBP1-89871) (0)

There are no publications for IL-10 ELISA Kit (NBP1-89871).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-10 ELISA Kit (NBP1-89871) (0)

There are no reviews for IL-10 ELISA Kit (NBP1-89871). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 ELISA Kit (NBP1-89871). (Showing 1 - 1 of 1 FAQ).

  1. Could you let me know whether you have used baculovirus to produce this product? 
    • For this kit, all proteins are expressed in an E.coli expression system. None of the components are expressed in baculovirus.

Additional IL-10 Products

Bioinformatics Tool for IL-10 ELISA Kit (NBP1-89871)

Discover related pathways, diseases and genes to IL-10 ELISA Kit (NBP1-89871). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-10 ELISA Kit (NBP1-89871)

Discover more about diseases related to IL-10 ELISA Kit (NBP1-89871).

Pathways for IL-10 ELISA Kit (NBP1-89871)

View related products by pathway.

PTMs for IL-10 ELISA Kit (NBP1-89871)

Learn more about PTMs related to IL-10 ELISA Kit (NBP1-89871).

Research Areas for IL-10 ELISA Kit (NBP1-89871)

Find related products by research area.

Blogs on IL-10.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Customers Who Bought This Also Bought

IL-10 [Unconjugated]

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-10 ELISA Kit (Colorimetric) and receive a gift card or discount.


Gene Symbol IL10