Human IL-10 ELISA Kit (Colorimetric)

Images

 
ELISA: IL-10 ELISA Kit [NBP1-89871] - Representative standard curve for human IL-10 ELISA. Human IL-10 was diluted in serial 2-fold steps in Assay Buffer (1x)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (citrate, heparin)
Standard Curve Range
3.15 - 200 pg/mL
Sensitivity
1.0 pg/mL

Order Details

Human IL-10 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-10 positive serum. There was no crossreactivity detected.
Standard Curve Range
3.15 - 200 pg/mL
Sensitivity
1.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.6%
Intra-Assay
3.2%
Spike Recovery
The recovery ranged from 81% to 106% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL10

Applications/Dilutions

Application Notes
The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-10.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-10
  2. Amplification Reagent II
  3. Amplification Diluent Concentrate (2x)
  4. Amplification Reagent I*
  5. Adhesive Films
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Biotin-Conjugate anti-human IL-10 monoclonal antibody
  8. * reagent contains ethyl alcohol
  9. Human IL-10 Standard lyophilized, 400 pg/mL upon reconstitution
  10. Control, lyophilized
  11. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  12. Substrate Solution (tetramethyl-benzidine)
  13. Streptavidin-HRP
  14. Stop Solution (1M Phosphoric acid)
  15. Sample Diluent

Alternate Names for Human IL-10 ELISA Kit (Colorimetric)

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL10
Entrez