Novus Biologicals products are now on

Human IL-10 Quantikine ELISA Kit

Product Details

Reactivity HuSpecies Glossary
Applications ELISA

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Human IL-10 Quantikine ELISA Kit Summary

The Quantikine Human IL-10 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-10 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10 and does not cross-react with viral IL-10. Resu...lts obtained using natural human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate this kit can be used to determine relative mass values for natural human IL-10.
Show More
Natural and recombinant human IL-10
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using
D1000B in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-10 Quantikine ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for IL-10 (D1000B)(207)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bovine, Complex Species Category, Primate - Pan troglodytes (Chimpanzee), Primate - Papio hamadryas (Hamadyras Baboon), Rabbit, Virus.

We have publications tested in 1 application: ELISA Capture.

Filter By Application
ELISA Capture
All Applications
Filter By Species
Complex Species Category
Primate - Pan troglodytes (Chimpanzee)
Primate - Papio hamadryas (Hamadyras Baboon)
All Species
Showing Publications 1 - 10 of 207. Show All 207 Publications.
Publications using D1000B Applications Species
Park, YJ;Acosta, D;Rubel Hoq, M;Khurana, S;Golding, H;Zaitseva, M; Pyrogenic and inflammatory mediators are produced by polarized M1 and M2 macrophages activated with D-dimer and SARS-CoV-2 spike immune complexes Cytokine 2024-01-01 [PMID: 38041875] (Human) Human
Zou, Y;Guo, S;Liao, Y;Chen, W;Chen, Z;Chen, J;Wen, L;Xie, X; Ceramide metabolism-related prognostic signature and immunosuppressive function of ST3GAL1 in osteosarcoma Translational oncology 2023-11-28 [PMID: 38029509] (Human) Human
Ur Rahim, J;Faheem, MM;Nawaz, S;Goswami, A;Rai, R; Synthesis and characterization of piperic acid conjugates with homochiral and heterochiral dipeptides containing phenylalanine and their application in skin cancer Peptides 2023-11-03 [PMID: 37923167] (Mouse) Mouse
Tomaszewski, M;Mertowska, P;Janczewska, M;Stycze?, A;Mertowski, S;Jonas, K;Grywalska, E;Kope?, G; In the Search for Biomarkers of Pulmonary Arterial Hypertension, Are Cytokines IL-2, IL-4, IL-6, IL-10, and IFN-Gamma the Right Indicators to Use? International journal of molecular sciences 2023-09-05 [PMID: 37761997] (Human) Human
Heo, SJ;Jee, YS; Intensity-effects of strengthening exercise on thigh muscle volume, pro- or anti-inflammatory cytokines, and immunocytes in the older adults: A randomized controlled trial Archives of gerontology and geriatrics 2023-07-23 [PMID: 37541052] (Human) Human
A Alshalani, BM Beuger, AM Tuip-de Bo, R van Brugge, JP Acker, NP Juffermans The impact of biological age of red blood cell on in vitro endothelial activation markers Frontiers in Physiology, 2023-03-08;14(0):1127103. 2023-03-08 [PMID: 36969576] (Human) Human
S Kameishi, CM Dunn, M Oka, K Kim, YK Cho, SU Song, DW Grainger, T Okano Rapid and effective preparation of clonal bone marrow-derived mesenchymal stem/stromal cell sheets to reduce renal fibrosis Scientific Reports, 2023-03-17;13(1):4421. 2023-03-17 [PMID: 36932137] (Human) Human
AG Saleh, AY Shehab, R Abdul-Ghan, AF Allam, MM Osman, HS Ibrahim, HA El-Taweel, EA Moneer, NA Hagras, HF Farag Key cytokines and hematological parameters in patients with uncomplicated falciparum malaria in Hodeidah, Yemen Cytokine, 2023-02-27;164(0):156156. 2023-02-27 [PMID: 36857851] (Human) Human
J Ferreira, M Oliveira, M Bicho, F Serejo Role of Inflammatory/Immune Response and Cytokine Polymorphisms in the Severity of Chronic Hepatitis C (CHC) before and after Direct Acting Antiviral (DAAs) Treatment International Journal of Molecular Sciences, 2023-01-10;24(2):. 2023-01-10 [PMID: 36674897] (Human) Human
M Babi? Leko, M Mihel?i?, J Jurasovi?, M Nikolac Pe, E Špani?, A Sekovani?, T Orct, K Zub?i?, L Langer Hor, N Plei?, S Ki?emet-Pi, Ž Vogrinc, N Pivac, A Diana, F Borove?ki, PR Hof, G Šimi? Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer's Disease International Journal of Molecular Sciences, 2022-12-27;24(1):. 2022-12-27 [PMID: 36613911] (Human) Human
Show All 207 Publications.

Reviews for IL-10 (D1000B) (0)

There are no reviews for IL-10 (D1000B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 (D1000B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-10 Products

Blogs on IL-10.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-10 Quantikine ELISA Kit and receive a gift card or discount.


Gene Symbol IL10