IL-10 Antibodies

417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417). The ND₅₀ is typically 0.005-0.015 µg/mL." class="big_lightbox"> 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417). The ND₅₀ is typically 0.005-0.015 µg/mL." class="big_thumb" />

417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_lightbox"> 522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217). At 2 µg/mL, this antibody will neutralize >60% Recombinant Human IL-10 induced bioactivity." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217). At 2 µg/mL, this antibody will neutralize >60% Recombinant Human IL-10 induced bioactivity." class="big_thumb" />

HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_thumb" />

HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171). The ND₅₀ is typically 0.005-0.03 µg/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171). The ND₅₀ is typically 0.005-0.03 µg/mL." class="big_thumb" />

117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_lightbox"> 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND₅₀ is typically 0.15-0.9 µg/mL." alt="Recombinant Viral HCMV IL-10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_thumb" />

522-RLB), Recombinant Human IL-10 (Catalog # 217-IL) and Recombinant Mouse IL-10 (Catalog # 417-ML). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Rat IL-10 Monoclonal Antibody (Catalog # MAB519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 522-RLB), Recombinant Human IL-10 (Catalog # 217-IL) and Recombinant Mouse IL-10 (Catalog # 417-ML). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Rat IL-10 Monoclonal Antibody (Catalog # MAB519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Rat IL-10 (Catalog # 522-RLB), Recombinant Human IL-10 (Catalog # 217-IL) and Recombinant Mouse IL-10 (Catalog # 417-ML). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Rat IL-10 Monoclonal Antibody (Catalog # MAB519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA). The ND₅₀ is typically 3-15 µg/mL." class="big_thumb" />

HAF018). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media from HEK293 human embryonic kidney cell line either mock transfected or transfected human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9184) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9184). The ND₅₀ is typically 15-90 ng/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9184). The ND₅₀ is typically 15-90 ng/mL." class="big_thumb" />